General Information
- Analysis ID
- OEZ008241
- Analysis Name
- MS data analysis
- Description
- Parameters of MaxQuant was set as follows: PIF (precursor intensity fraction) filter value – 0.75, reporter mass tolerance – 0.003 Da, fixed modification – Carbamidomethyl cysteine, variable modifications – Oxidized methionine, protein N-term acetylation, lysine, acetylation, asparagine and glutamine (NQ) deamidation (phospho (STY) added in iCCA phosphorylation data analysis), maximum number of modifications – 5, enzyme specificity – trypsin, maximum missing cleavage site – 2, tolerances of first search – 20 ppm, tolerances of main search – 4.5 ppm, minimal peptide length – 7, FDR (false discovery rates) of peptide, protein and site –0.01. The kit LOT number was used to corrected the purities of TMT channels for each set of TMT labeling data. The highly reliable sites (The Class I phosphorylation sites) will be considered if the localization probability filter >0.75.
Analysis Information
- Analysis Type
- Other
- Pipeline
Target
Analysis Data
Submitter Information
- Create Date
- 2021-12-30
- Last Modified
- 2021-12-31
- Submission
- Liangqing Dong
- Zhongshan Hospital, Fudan University