General Information
- Experiment ID
- OEX000016
- Experiment Name
- Hi-C sequencing
- Experiment Type
- Genomic
- Description
- The genomic DNA from exponential phase cells was cross-linked and digested with 200 unit MboI enzyme (NEB) as previously described36. The cutting DNA ends were labelled with biotin-14-dCTP (TriLINK) followed by ligation. Purified DNA was sheared to a length of ~400 bp. Point ligation junctions were pulled down with Dynabeads MyOne Streptavidin C1 (Thermofisher). The Hi-C library for Illumina sequencing was prepped with the NEBNext Ultra II DNA library Prep Kit for Illumina (NEB) according to the manufacturers’ instructions. Fragments between 400 and 600 bp were paired-end sequenced on a HiSeq2000 platform (Illumina).
Experiment information
- Attributes
-
library_strategy Hi-C library_layout Paired mate_pair n/a platform HiSeq X Ten library_name HiC - Project
-
1
Project ID Project name Create Date OEP000104 Creating a functional single-chromosome yeast 2018-03-11 - Samples
-
5
Sample ID Sample Name Organism Tissue Create Date OES002930 SY14 Saccharomyces cerevisiae N/A 2018-03-11 OES000008 SY13 Saccharomyces cerevisiae N/A 2018-04-24 OES000009 SY6 Saccharomyces cerevisiae N/A 2018-04-24 OES002929 WT Saccharomyces cerevisiae N/A 2018-03-11 OES013413 SY15 Saccharomyces cerevisiae N/A 2018-10-30 - Runs
- 5
Author Information
- Create Date
- 2018-04-24
- Last Modified
- 2018-12-26
- Submission
- Xiaoli Xue
- SIBS