General Information
- Experiment ID
- OEX000019
- Experiment Name
- OEX_Lan_1804271132
- Experiment Type
- Transcriptomic
- Description
- Genetic depletion of transcription factor Sox30 using CRISPR-Cas9 gene editing technology leads to defects in spermiogenesis, and uniformly arrested at early round spermatids. Specifically, in the absence of Sox30, proacrosomic vesicles fail to form a single acrosomal organelle. This is correlated with changes in postmeiotic gene expression. 1. Testes were collected from male wild-type mice at postnatal day 21 (P21), and testes depleted of Sox30 were collected from male Sox30 KO mice at P21. 2. Pachytene spermatocytes (PS) and round spermatids (RS) were isolated from wild-type (n=6)and Sox30 KO (n=8) mice at 8-week-old.
- Protocol
- mRNA was extracted from mouse testes samples and were subjected to RNA-seq. For wild-type group, we have three samples; for Sox30 KO group, we have three samples. mRNA was extracted from isolated PS and RS from wild-type and Sox30 KO mice at 8-week-old, respectively. Four samples : WT PS, Sox30 KO PS, WT RS, Sox30 KO RS
Experiment information
- Attributes
-
library_strategy RNA-Seq library_layout n/a library_construction n/a library_selection n/a mate_pair n/a platform HiSeq X Ten library_name n/a - Project
-
1
Project ID Project name Create Date OEP000012 Transcription factor Sox30 during germ cell development 2018-04-27 - Samples
-
10
Sample ID Sample Name Organism Tissue Create Date OES000055 Testis_WT_rep1_RNAseq Mus musculus testicle 2018-04-27 OES000056 Testis_WT_rep2_RNAseq Mus musculus testicle 2018-04-27 OES000057 Testis_WT_rep3_RNAseq Mus musculus testicle 2018-04-27 OES000058 Testis_KO_rep1_RNAseq Mus musculus testicle 2018-04-27 OES000059 Testis_KO_rep2_RNAseq Mus musculus testicle 2018-04-27 OES000060 Testis_KO_rep3_RNAseq Mus musculus testicle 2018-04-27 OES000061 PS_WT_RNAseq Mus musculus testicle 2018-04-27 OES000062 PS_KO_RNAseq Mus musculus testicle 2018-04-27 OES000063 RS_WT_RNAseq Mus musculus testicle 2018-04-27 OES000064 RS_KO_RNAseq Mus musculus testicle 2018-04-27 - Runs
-
10
Run ID Run Name Sample Data Num Create Date OER000137 OER_Lan_1804271347 Testis_WT_rep1_RNAseq 1 2018-04-27 OER000138 OER_Lan_1804271409 Testis_WT_rep2_RNAseq 1 2018-04-27 OER000139 OER_Lan_1804271411 Testis_WT_rep3_RNAseq 1 2018-04-27 OER000140 OER_Lan_1804271412 Testis_KO_rep1_RNAseq 1 2018-04-27 OER000141 OER_Lan_1804271414 Testis_KO_rep2_RNAseq 1 2018-04-27 OER000142 OER_Lan_1804271415 Testis_KO_rep3_RNAseq 1 2018-04-27 OER000143 OER_Lan_1804271513 PS_WT_RNAseq 1 2018-04-27 OER000144 OER_Lan_1804271516 PS_KO_RNAseq 1 2018-04-27 OER000145 OER_Lan_1804271516 RS_WT_RNAseq 1 2018-04-27 OER000146 OER_Lan_1804271519 RS_KO_RNAseq 1 2018-04-27
Author Information
- Create Date
- 2018-04-27
- Last Modified
- 2019-05-30
- Submission
- Lan Ye
- Nanjing Medical University