General Information
- Experiment ID
- OEX001547
- Experiment Name
- BE3ZEW
- Experiment Type
- Genomic
- Protocol
- The mixture of Cre, Cas9/BE3/ABE7.10 and sgRNA was injected into one blastomere of a 2-cell embryo, derived from Ai9 male mice mating with wild-type female mice. The action of Cre is expected to generate a chimeric embryo with half of cells labeled with tdTomato. The tdTomato+ cells and tdTomato- cells were isolated from the chimeric embryo at E14.5 by FACS and used for WGS respectively. The off-target SNVs and indels were identified by comparing the tdTomato+ cells with tdTomato- cells using three variant calling algorithms as indicated (Mutect2, Lofreq and Strelka for SNVs, and Mutect2, Scalpel and Strelka for indels).
Experiment information
- Attributes
-
library_strategy WGS library_layout Paired library_selection PCR mate_pair N platform Illumina HiSeq 2500 - Project
-
1
Project ID Project name Create Date OEP000195 Cytosine base editor generates substantial off-target single nucleotide variants in mouse embryos 2018-11-30 - Samples
-
22
Sample ID Sample Name Organism Tissue Create Date OES013948 B15 Mus musculus N/A 2018-11-30 OES013949 B16 Mus musculus N/A 2018-11-30 OES013950 SRS4025808 Mus musculus N/A 2018-11-30 OES013951 SRS4025809 Mus musculus N/A 2018-11-30 OES013952 SRS4025806 Mus musculus N/A 2018-11-30 OES013953 SRS4025807 Mus musculus N/A 2018-11-30 OES013954 B23 Mus musculus N/A 2018-11-30 OES013955 B24 Mus musculus N/A 2018-11-30 OES013956 B25 Mus musculus N/A 2018-11-30 OES013957 B26 Mus musculus N/A 2018-11-30 OES013958 B29 Mus musculus N/A 2018-11-30 OES013959 B30 Mus musculus N/A 2018-11-30 OES013960 B33 Mus musculus N/A 2018-11-30 OES013961 B34 Mus musculus N/A 2018-11-30 OES013962 SRS4025812 Mus musculus N/A 2018-11-30 OES013963 SRS4025813 Mus musculus N/A 2018-11-30 OES013964 SRS4025810 Mus musculus N/A 2018-11-30 OES013965 SRS4025811 Mus musculus N/A 2018-11-30 OES013966 SRS4025814 Mus musculus N/A 2018-11-30 OES013967 SRS4025815 Mus musculus N/A 2018-11-30 OES013968 SRS4025804 Mus musculus N/A 2018-11-30 OES013969 SRS4025805 Mus musculus N/A 2018-11-30 - Runs
-
22
Run ID Run Name Sample Data Num Create Date OER025228 B15 B15 2 2018-11-30 OER025229 B16 B16 2 2018-11-30 OER025230 SRR8169137 SRS4025808 2 2018-11-30 OER025231 SRR8169136 SRS4025809 2 2018-11-30 OER025232 SRR8169139 SRS4025806 2 2018-11-30 OER025233 SRR8169138 SRS4025807 2 2018-11-30 OER025234 B23 B23 2 2018-11-30 OER025235 B24 B24 2 2018-11-30 OER025236 B25 B25 2 2018-11-30 OER025237 B26 B26 2 2018-11-30 OER025238 B29 B29 2 2018-11-30 OER025239 B30 B30 2 2018-11-30 OER025240 B33 B33 2 2018-11-30 OER025241 B34 B34 2 2018-11-30 OER025242 SRR8169133 SRS4025812 2 2018-11-30 OER025243 SRR8169132 SRS4025813 2 2018-11-30 OER025244 SRR8169135 SRS4025810 2 2018-11-30 OER025245 SRR8169134 SRS4025811 2 2018-11-30 OER025246 SRR8169131 SRS4025814 2 2018-11-30 OER025247 SRR8169130 SRS4025815 2 2018-11-30 OER025248 SRR8169141 SRS4025804 2 2018-11-30 OER025249 SRR8169140 SRS4025805 2 2018-11-30 - Analysis
-
22
Analysis ID Analysis Title Analysis Type Data Num Create Date OEZ000416 B15 Other 2 2018-12-19 OEZ000417 B16 Other 4 2018-12-19 OEZ000418 B17 Other 6 2018-12-19 OEZ000419 B18 Other 8 2018-12-19 OEZ000420 B19 Other 10 2018-12-19 OEZ000421 B20 Other 12 2018-12-19 OEZ000422 B23 Other 14 2018-12-19 OEZ000423 B24 Other 16 2018-12-19 OEZ000424 B25 Other 18 2018-12-19 OEZ000425 B26 Other 20 2018-12-19 OEZ000426 B29 Other 22 2018-12-19 OEZ000427 B30 Other 24 2018-12-19 OEZ000428 B33 Other 26 2018-12-19 OEZ000429 B34 Other 28 2018-12-19 OEZ000454 s180904001WL Other 30 2018-12-19 OEZ000455 s180904002WL Other 32 2018-12-19 OEZ000456 s180904003WL Other 34 2018-12-19 OEZ000457 s180904004WL Other 36 2018-12-19 OEZ000458 s180904005WL Other 38 2018-12-19 OEZ000459 s180904006WL Other 40 2018-12-19 OEZ000460 s180904007WL Other 42 2018-12-19 OEZ000461 s180904008WL Other 44 2018-12-19
Author Information
- Create Date
- 2018-11-30
- Last Modified
- 2019-04-25
- Submission
- Hui Yang
- Shanghai Institutes for biological science, chinese academy of science