General Information
- Experiment ID
- OEX001577
- Experiment Name
- EMCV infection HeLa cell
- Experiment Type
- Transcriptomic
- Description
- HeLa cell with or without EMCV infection. Poly(A)+ RNA-seq for gene expression, poly(A)–/Ribo– for circRNA.
- Protocol
- HeLa cells (Human cervical cancer cells) were cultured with DMEM medium supplemented with 10% FBS; PA-1 (human ovarian adenocarcinoma cell) were cultured with MEMα medium supplemented with 10% FBS. For EMCV stimulation, HeLa cells (Human cervical cancer cells) were infected with EMCV (0.1 moi) for 24h.
Experiment information
- Attributes
-
library_strategy RNA-Seq library_layout Paired library_construction n/a library_selection n/a mate_pair n/a platform NextSeq 500 insertion_size 150 library_name EMCV infection - Project
-
1
Project ID Project name Create Date OEP000216 Structure and degradation of circular RNAs regulate PKR activation in innate immunity 2019-01-19 - Samples
- 4
- Runs
-
8
Run ID Run Name Sample Data Num Create Date OER025788 HeLa WT poly(A)+ rep2 HeLa WT poly(A)+ 2 2019-01-28 OER025789 HeLa WT poly(A)+ rep1 HeLa WT poly(A)+ 2 2019-01-28 OER025790 HeLa WT poly(A)– rep2 HeLa WT poly(A)– 2 2019-01-28 OER025791 HeLa WT poly(A)– rep1 HeLa WT poly(A)– 2 2019-01-28 OER025792 HeLa EMCV poly(A)+ rep2 HeLa EMCV poly(A)+ 2 2019-01-28 OER025793 HeLa EMCV poly(A)+ rep1 HeLa EMCV poly(A)+ 2 2019-01-28 OER025794 HeLa EMCV poly(A)– rep2 HeLa EMCV poly(A)– 2 2019-01-28 OER025795 HeLa EMCV poly(A)– rep1 HeLa EMCV poly(A)– 2 2019-01-28 - Analysis
-
8
Analysis ID Analysis Title Analysis Type Data Num Create Date OEZ004321 HeLa WT ctrl poly(A)+ RNA rep1 Reference Alignment 0 2019-01-28 OEZ004323 HeLa EMCV poly(A)+ RNA rep1 Reference Alignment 0 2019-01-28 OEZ004324 HeLa EMCV poly(A)+ RNA rep2 Reference Alignment 0 2019-01-28 OEZ004325 HeLa WT ctrl poly(A)– RNA rep1 Reference Alignment 0 2019-01-28 OEZ004326 HeLa WT ctrl poly(A)– RNA rep2 Reference Alignment 0 2019-01-28 OEZ004327 HeLa EMCV poly(A)– RNA rep1 Reference Alignment 0 2019-01-28 OEZ004328 HeLa EMCV poly(A)– RNA rep2 Reference Alignment 0 2019-01-28 OEZ004329 HeLa WT ctrl poly(A)+ RNA rep2 Reference Alignment 0 2019-01-29
Author Information
- Create Date
- 2019-01-19
- Last Modified
- 2019-04-16
- Submission
- Li Yang
- CAS-MPG Partner Institute for Computational Biology