- Experiment ID
- Experiment Name
- SLE patient and normal donors RNA-seq data
- Experiment Type
- Ribosomal RNA-depleted RNAs from three major mononuclear cell populations including monocytes, B cells and T cells isolated from PBMCs of pooled SLE patients and normal donors for RNA-seq.
- Individual PBMCs were isolated by density-gradient centrifugation with Ficoll-Paque Premium. Then CD14+ monocytes, CD19+ B cells and CD3+ T cells from individual PBMCs were separated using CD14, CD19 or CD3 microbeads (Miltenyi Biotec) according to the manufacturer’s instructions. Isolated T cells, monocytes and B cells were collected for using or storage in liquid nitrogen. Total RNA were depleted rRNA during preparing library for RNA-seq. Normal RNA-seq data were from 7 donors mixed samples . SLE patient RNA-seq samples were mixed by 10 donors.
library_strategy RNA-Seq library_layout Paired library_construction n/a library_selection n/a mate_pair n/a platform NextSeq 500 insertion_size 150 library_name n/a
Project ID Project name Create Date OEP000216 Structure and degradation of circular RNAs regulate PKR activation in innate immunity 2019-01-19
Sample ID Sample Name Organism Tissue Create Date OES014927 Monoctye normal donors Homo sapiens N/A 2019-02-15 OES014928 Monocyte SLE patient Homo sapiens N/A 2019-02-15 OES014929 Bcell normal donors Homo sapiens B cell 2019-02-15 OES014930 Bcell SLE patient Homo sapiens B cell 2019-02-15 OES014932 Tcell SLE patient Homo sapiens T cell 2019-02-15 OES014931 Tcell normal donors Homo sapiens T cell 2019-02-15
Run ID Run Name Sample Data Num Create Date OER027277 Monoctye_normal_ribo(–) Monoctye normal donors 2 2019-02-15 OER027278 Monocyte_SLE_ribo(–) Monocyte SLE patient 2 2019-02-15 OER027279 Bcell_normal_ribo(–) Bcell normal donors 2 2019-02-15 OER027280 Bcell_SLE_ribo(–) Bcell SLE patient 2 2019-02-15 OER027281 Tcell_SLE_ribo(–) Tcell SLE patient 2 2019-02-15 OER027282 Tcell_normal_ribo(–) Tcell normal donors 2 2019-02-15
Analysis ID Analysis Title Analysis Type Data Num Create Date OEZ004333 Monocyte normal Reference Alignment 0 2019-02-15 OEZ004334 Monocyte SLE patient Reference Alignment 0 2019-02-15 OEZ004335 Bcell normal donor Reference Alignment 0 2019-02-15 OEZ004336 Bcell SLE patient Reference Alignment 0 2019-02-15 OEZ004337 Tcell normal donors Reference Alignment 0 2019-02-15 OEZ004338 Tcell SLE patient Reference Alignment 0 2019-02-15
- Create Date
- Last Modified
- Li Yang
- CAS-MPG Partner Institute for Computational Biology