General Information
- Experiment ID
- OEX002247
- Experiment Name
- Genomic
- Experiment Type
- Genomic
- Description
- ChIP-Seq
- Protocol
- Nuclei from formaldehyde crosslinked (1%; 10min at room temperature) embryos were isolated and chromatin was fragmented using sonicator (Branson). Lysates were cleared and protein-DNA complexes were isolated using target specific antibodies and Dynabeads. DNA fragments were purified and libraries were prepared by NEBnext DNA Library Prep Kit (NEB, E7370L). Adapter ligated DNA was purified using AMPure XP beads (Beckman Coulter, A63881) and sequenced by illumina HiSeq 2500. NEBnext DNA Library Prep Kit
Experiment information
- Attributes
-
library_strategy ChIP-Seq library_selection ChIP library_layout Single mate_pair N platform Illumina HiSeq 2500 library_name ChIP-seq - Project
-
1
Project ID Project name Create Date OEP000775 test step by step multipel project 2020-02-28 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES025991 10012_tumor Homo sapiens liver 2020-02-28 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER039856 10012_T 10012_tumor 2 2020-02-28 - Analysis
-
1
Analysis ID Analysis Title Analysis Type Data Num Create Date OEZ005406 variation Other 0 2020-02-28
Author Information
- Create Date
- 2020-02-28
- Last Modified
- 2024-03-08
- Submission
- node_dev node_dev
- Shanghai Institute of Nutrition ang Health, CAS-MPG Partner Institute for Computational Biology