General Information
- Experiment ID
- OEX011295
- Experiment Name
- Glycine metabolism RNA-seq
- Experiment Type
- Transcriptomic
- Protocol
- After the total RNA extraction and DNase I treatment, magnetic beads with Oligo (dT) are used to isolate mRNA(for eukaryotes) or by removing rRNAs from the total RNA (for prokaryotes). Mixed with the fragmentation buffer, the mRNA is fragmented into short fragments. Then cDNA is synthesized using the mRNA fragments as templates. Short fragments are purified and resolved with EB buffer for end reparation and single nucleotide A (adenine) addition. After that, the short fragments are connected with adapters. The suitable fragments are selected for the PCR amplification as templates. During the QC steps, Agilent 2100 Bioanaylzer and ABI StepOnePlus Real-Time PCR System are used in quantification and qualification of the sample library. At last, the library could be sequenced using BGISEQ-500.
Experiment information
- Attributes
-
library_layout Paired mate_pair n/a insertion_size n/a number_of_reads_sequenced n/a library_name n/a library_selection RANDOM PCR library_strategy RNA-Seq platform BGISEQ-500 - Project
-
1
Project ID Project name Create Date OEP001473 Glycine deprivation induces DNA damage and thereby results in cell cycle arrest in myeloma cells 2021-01-08 - Samples
-
14
Sample ID Sample Name Organism Tissue Create Date OES060195 Glycine_0mg_6hA1 Homo sapiens BoneMarrow 2021-01-09 OES060196 Glycine_0mg_6h_A2 Homo sapiens BoneMarrow 2021-01-09 OES060197 Glycine_0mg_12h_A1 Homo sapiens BoneMarrow 2021-01-09 OES060198 Glycine_0mg_12h_A2 Homo sapiens BoneMarrow 2021-01-09 OES060199 Glycine_0mg_24h_A1 Homo sapiens BoneMarrow 2021-01-09 OES060200 Glycine_0mg_24h_A2 Homo sapiens BoneMarrow 2021-01-09 OES060201 Glycine_10mg_6h_A1 Homo sapiens BoneMarrow 2021-01-09 OES060202 Glycine_10mg_6h_A2 Homo sapiens BoneMarrow 2021-01-09 OES060203 Glycine_10mg_12h_A1 Homo sapiens BoneMarrow 2021-01-09 OES060204 Glycine_10mg_12h_A2 Homo sapiens BoneMarrow 2021-01-09 OES060205 Glycine_10mg_24h_A1 Homo sapiens BoneMarrow 2021-01-09 OES060206 Glycine_10mg_24h_A2 Homo sapiens BoneMarrow 2021-01-09 OES060207 Normal_0h_A1 Homo sapiens BoneMarrow 2021-01-09 OES060208 Normal_0h_A2 Homo sapiens BoneMarrow 2021-01-09 - Runs
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14
Run ID Run Name Sample Data Num Create Date OER094489 Glycine 0mg 6h A1 Glycine_0mg_6hA1 2 2021-01-09 OER094490 Glycine 0mg 6h A2 Glycine_0mg_6h_A2 2 2021-01-09 OER094491 Glycine 0mg 12h A1 Glycine_0mg_12h_A1 2 2021-01-09 OER094492 Glycine 0mg 12h A2 Glycine_0mg_12h_A2 2 2021-01-09 OER094493 Glycine 0mg 24h A1 Glycine_0mg_24h_A1 2 2021-01-09 OER094494 Glycine 0mg 24h A2 Glycine_0mg_24h_A2 2 2021-01-09 OER094495 Glycine 10mg 6h A1 Glycine_10mg_6h_A1 2 2021-01-09 OER094496 Glycine 10mg 6h A2 Glycine_10mg_6h_A2 2 2021-01-09 OER094497 Glycine 10mg 12h A1 Glycine_10mg_12h_A1 2 2021-01-09 OER094498 Glycine 10mg 12h A2 Glycine_10mg_12h_A2 2 2021-01-09 OER094499 Glycine 10mg 24h A1 Glycine_10mg_24h_A1 2 2021-01-09 OER094500 Glycine 10mg 24h A2 Glycine_10mg_24h_A2 2 2021-01-09 OER094501 Normal 0h A1 Normal_0h_A1 2 2021-01-09 OER094502 Normal 0h A2 Normal_0h_A2 2 2021-01-09
Author Information
- Create Date
- 2021-01-08
- Last Modified
- 2023-12-01
- Submission
- Zhenhao Liu
- SCBIT