General Information
- Experiment ID
- OEX014793
- Experiment Name
- OEP002901_HD_scRNAseq
- Experiment Type
- Transcriptomic single cell
- Description
- Single-cell RNA sequencing of sorted HD donors decidual CD45+ and CD45- cells and mixed at a ratio of 2:1 and subjected to scRNA-seq using the 10× Genomics platform.
- Protocol
- On arrival, freshly human decidual tissues were transferred rapidly to a 10-cm dish and washed twice with 1x cold PBS(Gibco). Each tissue was trimmed into 1-mm3 pieces and digested for 45 min each at 37℃ with medium containing 1 mg/mL collagenase IV (Gibcol, 17104019) and 1 U/mL dispase II (Gibcol, 17105041). The cell suspensions were filtered through a 40-μm cell-strainer nylon mesh(BD) and were collected by centrifuging at 700 × g for 10 min. Dissociated cells were washed with MACS buffer (PBS containing 1% FBS, 0.5% EDTA, and 0.05% gentamycin).
Experiment information
- Attributes
-
library_layout Paired mate_pair Yes library_selection cDNA library_strategy RNA-seq platform Illumina NovaSeq 6000 - Project
-
1
Project ID Project name Create Date OEP002901 The recurrent spontaneous abortion (RSA) patients' abnormal decidual microenvironment 2021-11-15 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES127986 HD_decidua Homo sapiens decidua tissue 2021-11-15 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER234682 1_HD HD_decidua 15 2021-11-15
Author Information
- Create Date
- 2021-11-15
- Last Modified
- 2023-04-26
- Submission
- Zechuan Chen
- Chinese Academy of Sciences