General Information
- Experiment ID
- OEX023068
- Experiment Name
- CYLiu_experiment_2 (WuT)
- Experiment Type
- Transcriptomic
- Description
- fter subjecting the anesthetized mice to heart perfusion with PBS to remove peripheral blood, the lymph nodes in the mammary tissue were extracted. Following this, the fat pads were isolated from 8-week-old nulliparous or other specified-stage female mice. Fat pads were minced and placed in appropriate volume (1 g/10 ml) digestion mix (RPMI 1640 with 25mM HEPES, 5% fetal bovine serum, 1% penicillin-streptomycin-glutamine, 300 U/ml type 3 collagenase (Worthington)) and digested for 2h in a 100 rpm/min shaker at 37 °C, give a good shake every 15min.
Experiment information
- Attributes
-
library_layout Paired mate_pair n/a insertion_size n/a number_of_reads_sequenced n/a library_name n/a library_selection cDNA library_strategy RNA-Seq platform HiSeq X Ten - Project
-
1
Project ID Project name Create Date OEP004107 Adult_Mammary glands_10x RNAseq_1 2023-04-14 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES305865 WuT_19X134_Ctrl Mus mammary gland 2023-04-14 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER394057 OER_2304141820 WuT_19X134_Ctrl 0 2023-04-14
Author Information
- Create Date
- 2023-04-14
- Last Modified
- 2023-04-14
- Submission
- Chunye Liu
- Shanghai Institutes for Biological Sciences