General Information
- Experiment ID
- OEX025490
- Experiment Name
- OEX_Cui-Cui_2311201516
- Experiment Type
- Genomic
- Description
- To validate our observation, we performed chromatin immunoprecipitation (ChIP) seq of H3K4me1/2/3 between control and EMSY group, and the result showed that there were high H3K4me2/3 occupancies in the key regulatory promoter regions of stem cell markers, such as NANOG, POU5F1 and SOX2, in EMSY-overexpressing cells.
- Protocol
- Briefly, 107 to 108 TNBC cells (Control or EMSY) were harvested, crosslinking was performed with 1% formaldehyde or 1% paraformaldehyde for 10 minutes. To enhance cell lysis, we ran the lysate through a 27g needle three times and flash froze it in −80°C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 20 minutes. Protein/DNA complex was precipitated by specific antibodies against H3K4me2/3 (CST) and IgG control (Millipore, Burlington, MA, USA). DNA was purified using DNA Purification Kit (Qiagen, Hilden, Germany). PCR was performed using Phusion High Fidelity PCR master mix. ChIP-enriched chromatins were sequenced on Illumina HiSeq 2000 platforms according to the manufacture’s protocol.
Experiment information
- Attributes
-
library_layout Paired mate_pair n/a insertion_size n/a number_of_reads_sequenced n/a library_name n/a library_selection ChIP library_strategy ChIP-Seq platform Illumina HiSeq 2000 - Project
-
1
Project ID Project name Create Date OEP004750 EMSY enhances cancer stem cell self-renewal and tumorigenesis by reshaping methionine metabolism in triple-negative breast cancer 2023-11-20 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES334707 OES-CUi-Cui-2243122 Cancer cell line 2023-11-20 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER440354 OER_Cui-Cui_2311201543 OES-CUi-Cui-2243122 1 2023-11-20
Author Information
- Create Date
- 2023-11-20
- Last Modified
- 2023-11-20
- Submission
- Cui-Cui Liu
- Shanghai cancer center