General Information
- Experiment ID
- OEX027252
- Experiment Name
- RNA sequencing upon SAT1 overexpression
- Experiment Type
- Transcriptomic
- Description
- To study the role of SAT1-mediated polyamine catabolism in lung cancer, we established a doxycycline inducible SAT1 expression (OE) lung adenocarcinoma (LUAD) A549 cell line. RNA sequencing was performed for SAT1 OE (dox treatment for 24 hours) and control cells to identify the significantly upregulated and downregulated genes caused by SAT1 OE.
- Protocol
- Total RNA was extracted from cells by Trizol reagent (Invitrogen) and then the RNA quality was assessed by Bioanalyzer 2100 (Agilent). The RNA with RIN (RNA integrity number) > 7.0 was used for cDNA library construction. mRNA-Seq library was prepared using Stranded mRNA-seq Library Prep Kit (Vazyme) following the manufacturer’s manual. Prepared libraries with different sequence indexes were pooled at equal molar and sequenced on Illumina X-ten using standard protocol.
- Related Links
- n/a n/a
Experiment information
- Attributes
-
library_layout Paired mate_pair No insertion_size 300-400 number_of_reads_sequenced n/a library_name n/a library_selection RT-PCR library_strategy RNA-Seq platform Illumina HiSeq 2500 - Project
-
1
Project ID Project name Create Date OEP005018 Activation of polyamine catabolism promotes glutamine metabolism and creates a targetable vulnerability in lung cancer 2024-01-24 - Samples
- 4
- Runs
- 4
Author Information
- Create Date
- 2024-01-24
- Last Modified
- 2024-01-25
- Submission
- Ning Wang
- Shanghai Institute of Nutrition and Health, CAS