General Information
- Experiment ID
- OEX027259
- Experiment Name
- Old_LSK_ATAC_seq
- Experiment Type
- Transcriptomic
- Description
- LSK (Lin-c-Kit+Sca1+) cells sorted from old Setd2 deficiency mice and control mice were used for ATAC sequencing.
- Protocol
- A total of 1×105 BM LSKs from WT and KO-MDS mice were sorted, washed in DPBS, and lysed in 50 μL lysis buffer (10 mM Tris-HCl pH 7.4, 10 mM NaCl, 3 mM MgCl2, 0.1% (v/v) Igepal CA-630) for the follow-up process, which was performed by TruePrepTM DNA Library Prep Kit V2 for Illumina (TD501, Vazyme) according to the manufacturer’s instructions. Subsequently, the library fragments were purified by VAHTS DNA clean beads (Vazyme, N411-01) for future sequencing according to standard illumina HiSeq/NextSeq platform manuscripts.
Experiment information
- Attributes
-
library_layout Paired mate_pair n/a insertion_size n/a number_of_reads_sequenced n/a library_name n/a library_selection PCR library_strategy ATAC-seq platform Illumina NovaSeq 6000 - Project
-
1
Project ID Project name Create Date OEP004811 Loss of Setd2 induces systemic inflammation via embryo-derived Kupffer-like cell differentiation to drive malignant transformation 2023-12-07 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES354765 Old_LSK_ATAC_seq Mus musculus bone marrow 2024-01-26 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER458861 Old_LSK_ATAC Old_LSK_ATAC_seq 4 2024-01-26
Author Information
- Create Date
- 2024-01-26
- Last Modified
- 2024-02-27
- Submission
- Yuanliang Zhang
- Shanghai Institute of Hematology