General Information
- Experiment ID
- OEX027260
- Experiment Name
- Old_RNA-seq
- Experiment Type
- Transcriptomic
- Description
- cells from WT and Setd2 deficiency mice were sorted for RNA sequencing.
- Protocol
- For bulk RNA-seq, total RNA samples were extracted, and ribosomal RNA (rRNA) was removed using the Ribo-Zero rRNA Removal Kit (MRZMB126, Illumina). Subsequently, rRNA-depleted samples were fragmented, then subjected to first and second strand cDNA synthetization. cDNAs were ligated with sequencing adapters according to standard Novaseq 6000 platform (Illumina) manuscripts.
Experiment information
- Attributes
-
library_layout Paired mate_pair n/a insertion_size n/a number_of_reads_sequenced n/a library_name n/a library_selection PCR library_strategy RNA-Seq platform Illumina NovaSeq 6000 - Project
-
1
Project ID Project name Create Date OEP004811 Loss of Setd2 induces systemic inflammation via embryo-derived Kupffer-like cell differentiation to drive malignant transformation 2023-12-07 - Samples
-
1
Sample ID Sample Name Organism Tissue Create Date OES354766 Old_RNA-seq Mus musculus bone marrow, liver 2024-01-26 - Runs
-
1
Run ID Run Name Sample Data Num Create Date OER458862 Old_RNA-seq Old_RNA-seq 32 2024-01-26
Author Information
- Create Date
- 2024-01-26
- Last Modified
- 2024-02-27
- Submission
- Yuanliang Zhang
- Shanghai Institute of Hematology