Notice: Private records are filtered that would not be displayed.
Sort:
  • EXPR OEX_zhang_2403291949

    zhangwen  xiao, , 2024.03.29

    Project ID

    OEP005172

    Project Name

    N/A

    Experiment Type

    Genomic

    Protocol

    n/a

    OEX027665

  • EXPR Transcriptomic

    Lan  Yu, , 2024.03.29

    Project ID

    OEP005161

    Project Name

    VLS 16S DATA in girls

    Experiment Type

    Transcriptomic

    OEX027664

  • EXPR H3K4me3 ChIP-seq for of CD4+T cells form SLE patient and healthy control

    Delong  Feng, The Second Xiangya hospital of Central South University, 2023.08.12

    Project ID

    OEP004403

    Project Name

    H3K4me3 ChIP-seq of CD4+T cell from SLE patients and HC

    Experiment Type

    Genomic

    Protocol

    ChIP-seq was performed as previously described. Briefly, 2-5 million CD4+ T cells were lysed in cell lysis buffer for 5 min on ice. Cell were pelleted and resuspened with 200 μl of digestion buffer. Digestion was then performed by adding micrococcal nuclease and incubation for 5 min at 37 °C. The reaction was stopped by EDTA-EGTA buffer . After centrifuge for 15 min at 13000rpm, 1/10 of the supernatant were taken as Input sample, others supernatant was dilute into a CHIP-IP buffer. Chromatin was then incubated overnight at 4 °C with anti-trimethylated H3K4 antibody . Protein G magnetic beads were added for an additional 4 h of incubation. Immunoprecipitates were washed vigorously and DNA was purified with PCR purification kit. 1ng-50ng H3K4me3 ChIP'ed DNA or input DNA was used for ChIP-seq library construction via TruePrep DNA Library Prep Kit V2 for Illumina. After PCR step, the DNA libraries were purified and size selection by 0.5× and 0.25× DNA beads. ChIP-seq libraries were sequenced via paired-end 150 bp reads on an Illumina platform.

    OEX024597

  • EXPR Transcriptomic

    Liu  Yabin, Shanghai Public Health Clinical Center, 2024.03.27

    Project ID

    OEP005165

    Project Name

    Screening and identification of immunological signatures in cord blood of HIV-exposed uninfected infants

    Experiment Type

    Transcriptomic

    OEX027448

  • EXPR OEX_Yuanyuan_2303272231

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022970

  • EXPR OEX_Yuanyuan_2303272232

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022971

  • EXPR OEX_Yuanyuan_2303272229

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022969

  • EXPR OEX_Yuanyuan_2303272223

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022968

  • EXPR Transcriptomic

    wenlian  Chen, Cancer institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2024.02.02

    Project ID

    OEP004978

    Project Name

    Unveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma

    Experiment Type

    Transcriptomic

    OEX027287

  • EXPR MeRIP-seq

    wenlian  Chen, Cancer institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2024.02.03

    Project ID

    OEP004978

    Project Name

    Unveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma

    Experiment Type

    Transcriptomic

    OEX027291