Project IDOEP005172
Project NameN/A
Experiment TypeGenomic
Protocoln/a
OEX027665
Project IDOEP005161
Project NameVLS 16S DATA in girls
Experiment TypeTranscriptomic
OEX027664
Project IDOEP004403
Project NameH3K4me3 ChIP-seq of CD4+T cell from SLE patients and HC
Experiment TypeGenomic
ProtocolChIP-seq was performed as previously described. Briefly, 2-5 million CD4+ T cells were lysed in cell lysis buffer for 5 min on ice. Cell were pelleted and resuspened with 200 μl of digestion buffer. Digestion was then performed by adding micrococcal nuclease and incubation for 5 min at 37 °C. The reaction was stopped by EDTA-EGTA buffer . After centrifuge for 15 min at 13000rpm, 1/10 of the supernatant were taken as Input sample, others supernatant was dilute into a CHIP-IP buffer. Chromatin was then incubated overnight at 4 °C with anti-trimethylated H3K4 antibody . Protein G magnetic beads were added for an additional 4 h of incubation. Immunoprecipitates were washed vigorously and DNA was purified with PCR purification kit. 1ng-50ng H3K4me3 ChIP'ed DNA or input DNA was used for ChIP-seq library construction via TruePrep DNA Library Prep Kit V2 for Illumina. After PCR step, the DNA libraries were purified and size selection by 0.5× and 0.25× DNA beads. ChIP-seq libraries were sequenced via paired-end 150 bp reads on an Illumina platform.
OEX024597
Project IDOEP005165
Project NameScreening and identification of immunological signatures in cord blood of HIV-exposed uninfected infants
Experiment TypeTranscriptomic
OEX027448
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022970
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022971
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022969
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022968
Project IDOEP004978
Project NameUnveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma
Experiment TypeTranscriptomic
OEX027287
Project IDOEP004978
Project NameUnveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma
Experiment TypeTranscriptomic
OEX027291