Project IDOEP004403
Project NameH3K4me3 ChIP-seq of CD4+T cell from SLE patients and HC
Experiment TypeGenomic
ProtocolChIP-seq was performed as previously described. Briefly, 2-5 million CD4+ T cells were lysed in cell lysis buffer for 5 min on ice. Cell were pelleted and resuspened with 200 μl of digestion buffer. Digestion was then performed by adding micrococcal nuclease and incubation for 5 min at 37 °C. The reaction was stopped by EDTA-EGTA buffer . After centrifuge for 15 min at 13000rpm, 1/10 of the supernatant were taken as Input sample, others supernatant was dilute into a CHIP-IP buffer. Chromatin was then incubated overnight at 4 °C with anti-trimethylated H3K4 antibody . Protein G magnetic beads were added for an additional 4 h of incubation. Immunoprecipitates were washed vigorously and DNA was purified with PCR purification kit. 1ng-50ng H3K4me3 ChIP'ed DNA or input DNA was used for ChIP-seq library construction via TruePrep DNA Library Prep Kit V2 for Illumina. After PCR step, the DNA libraries were purified and size selection by 0.5× and 0.25× DNA beads. ChIP-seq libraries were sequenced via paired-end 150 bp reads on an Illumina platform.
OEX024597
Project IDOEP005165
Project NameScreening and identification of immunological signatures in cord blood of HIV-exposed uninfected infants
Experiment TypeTranscriptomic
OEX027448
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022970
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022971
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022969
Project IDOEP004085
Project NameMetabolic characterization of sphere-derived prostate cancer stem cells
Experiment TypeMetabolomic
OEX022968
Project IDOEP004978
Project NameUnveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma
Experiment TypeTranscriptomic
OEX027287
Project IDOEP004978
Project NameUnveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma
Experiment TypeTranscriptomic
OEX027291
Project IDOEP005154
Project NameCarotid endarterectomy ameliorates cognitive impairment via restoring cerebral hemodynamic, metabolic, and white matter status in unilateral carotid artery stenosis
Experiment TypeTranscriptomic
OEX027440
Project IDOEP005133
Project NameCancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis
Experiment TypeTranscriptomic
ProtocolThe TRIzol method was used for the RNA extraction from the prostate cancer tissues. Novogene (Beijing, China) was used to construct the cDNA library. An Illumina NovaSeq 6000 platform (Illumina, Novogene) was used for RNA-seq. RNA-seq data were analyzed using the R package (DESeq).
OEX027424