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  • EXPR H3K4me3 ChIP-seq for of CD4+T cells form SLE patient and healthy control

    Delong  Feng, The Second Xiangya hospital of Central South University, 2023.08.12

    Project ID

    OEP004403

    Project Name

    H3K4me3 ChIP-seq of CD4+T cell from SLE patients and HC

    Experiment Type

    Genomic

    Protocol

    ChIP-seq was performed as previously described. Briefly, 2-5 million CD4+ T cells were lysed in cell lysis buffer for 5 min on ice. Cell were pelleted and resuspened with 200 μl of digestion buffer. Digestion was then performed by adding micrococcal nuclease and incubation for 5 min at 37 °C. The reaction was stopped by EDTA-EGTA buffer . After centrifuge for 15 min at 13000rpm, 1/10 of the supernatant were taken as Input sample, others supernatant was dilute into a CHIP-IP buffer. Chromatin was then incubated overnight at 4 °C with anti-trimethylated H3K4 antibody . Protein G magnetic beads were added for an additional 4 h of incubation. Immunoprecipitates were washed vigorously and DNA was purified with PCR purification kit. 1ng-50ng H3K4me3 ChIP'ed DNA or input DNA was used for ChIP-seq library construction via TruePrep DNA Library Prep Kit V2 for Illumina. After PCR step, the DNA libraries were purified and size selection by 0.5× and 0.25× DNA beads. ChIP-seq libraries were sequenced via paired-end 150 bp reads on an Illumina platform.

    OEX024597

  • EXPR Transcriptomic

    Liu  Yabin, Shanghai Public Health Clinical Center, 2024.03.27

    Project ID

    OEP005165

    Project Name

    Screening and identification of immunological signatures in cord blood of HIV-exposed uninfected infants

    Experiment Type

    Transcriptomic

    OEX027448

  • EXPR OEX_Yuanyuan_2303272231

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022970

  • EXPR OEX_Yuanyuan_2303272232

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022971

  • EXPR OEX_Yuanyuan_2303272229

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022969

  • EXPR OEX_Yuanyuan_2303272223

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.03.27

    Project ID

    OEP004085

    Project Name

    Metabolic characterization of sphere-derived prostate cancer stem cells

    Experiment Type

    Metabolomic

    OEX022968

  • EXPR Transcriptomic

    wenlian  Chen, Cancer institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2024.02.02

    Project ID

    OEP004978

    Project Name

    Unveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma

    Experiment Type

    Transcriptomic

    OEX027287

  • EXPR MeRIP-seq

    wenlian  Chen, Cancer institute, Longhua Hospital, Shanghai University of Traditional Chinese Medicine, 2024.02.03

    Project ID

    OEP004978

    Project Name

    Unveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma

    Experiment Type

    Transcriptomic

    OEX027291

  • EXPR Mouse_RNAseq

    Yuting  Dai, Shanghai Institute of Hematology, 2024.03.24

    Project ID

    OEP005154

    Project Name

    Carotid endarterectomy ameliorates cognitive impairment via restoring cerebral hemodynamic, metabolic, and white matter status in unilateral carotid artery stenosis

    Experiment Type

    Transcriptomic

    OEX027440

  • EXPR Transcriptome

    Zhi  Xiong, , 2024.03.15

    Project ID

    OEP005133

    Project Name

    Cancer-associated fibroblasts promote enzalutamide resistance and PD-L1 expression in prostate cancer through CCL5-CCR5 paracrine axis

    Experiment Type

    Transcriptomic

    Protocol

    The TRIzol method was used for the RNA extraction from the prostate cancer tissues. Novogene (Beijing, China) was used to construct the cDNA library. An Illumina NovaSeq 6000 platform (Illumina, Novogene) was used for RNA-seq. RNA-seq data were analyzed using the R package (DESeq).

    OEX027424