Jianxiang  Shi, Zhengzhou University, 2023.05.25

Project ID

OEP004164

Project Name

Identification of potential resistance mechanisms and therapeutic targets for the relapse of BCMA CAR-T therapy in relapsed/refractory multiple myeloma through single-cell sequencing

Experiment Type

Transcriptomic single cell

Protocol

Our group launched a single-arm, single-center clinical trial on BCMA CAR-T cells in the treatment of R/R-MM on April 11, 2018. The construction of anti-BCMA CAR, lentivirus production and production of CAR-T cells were described by our previous study [11]. Thirty-four patients with R/R-MM were treated with BCMA CAR-T cell therapy and the preliminary results have been reported in the journal of leukemia (ClinicalTrials.gov Identifier: NCT03661554) [11]. The ORR is 88.2% and the median progression-free survival time (PFS) is 12.1 months [11]. To analyze the mechanism of relapse after BCMA CAR-T cell therapy, one patient at baseline (R/R-MM) and one patient at progression (first relapse after BCMA CAR-T therapy) were enrolled in the study. The patient relapse after BCMA CAR-T cell therapy is from one of the 34 patients, who was enrolled in this clinical trial on December 13, 2019, and a relapse was detected on October 27, 2021(namely as progression in this study). And the other patient is from the newly enrolled, who met the criteria for enrollment in the clinical trial on December 3, 2021, after screening (namely as baseline in this study). The detailed characteristics of these two patients including clinicopathological features, bone marrow plasma cell ratio, staging and so on were comparable and similar (Additional file 1: Table S1). Five milliliters (mL) of bone marrow were then extracted from the two patients, and the CD45+ cells were enriched with human CD45 microbeads, according to the manufacturer’s instructions (Miltenyi Biotec, Cat#:130-045-801). The CD45+ cells were subsequently sent for 10 × genomics ScRNA-seq.

OEX023766

Qian  Yang, CAS, 2023.08.29

Project ID

OEP004488

Project Name

Mangrove plastisphere metagenome

Experiment Type

Metagenomic

OEX025067

Wenran  Li, , 2023.11.27

Project ID

OEP004768

Project Name

CF DNA methylation of blood tissue

Experiment Type

Microarray

OEX025508

Luo  Ruijin, , 2023.11.09

Project ID

OEP004707

Project Name

测试项目

Experiment Type

Genomic

OEX025373

maoxiang  xu, shanghai jiaotong university, 2022.06.09

Project ID

OEP003436

Project Name

glomus jugulare tumors

Experiment Type

Transcriptomic single cell

OEX016883

Chenxu  Gao, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital, 2023.03.28

Project ID

OEP004100

Project Name

Pancreatic ductal adenocarcinoma adjuvant chemotherapy vulnerability: a prospective observational study

Experiment Type

Proteomic

OEX022972

Chenxu  Gao, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital, 2023.11.24

Project ID

OEP004100

Project Name

Pancreatic ductal adenocarcinoma adjuvant chemotherapy vulnerability: a prospective observational study

Experiment Type

Transcriptomic

OEX025504

Yu  Zhang, Shanghai Jiaotong University, 2023.02.23

Project ID

OEP003879

Project Name

MT-Enviro

Experiment Type

Metagenomic

Protocol

NEBNext Ultra II FS DNA Prep Kit with Illumina adapters

OEX021340

Yu  Zhang, Shanghai Jiaotong University, 2023.02.23

Project ID

OEP003879

Project Name

MT-Enviro

Experiment Type

Metatranscriptomic

Protocol

Ribo-Zero™ rRNA Removal Kit; NEBNext® Ultra IITM Directional RNA Library Prep Kit for Illumina® with Illumina adapters

OEX021341

Yu  He, China University of Geosciences (Wuhan), 2023.07.19

Project ID

OEP004270

Project Name

Bacteriophage contributes folate-associated cofactors to microbial consortia implied by ultrabasic viruses in steel slag leachate

Experiment Type

Genomic

OEX024476