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  • EXPR OEX_Cui-Cui_2311201516

    Cui-Cui  Liu, Shanghai cancer center, 2023.11.20

    Project ID

    OEP004750

    Project Name

    EMSY enhances cancer stem cell self-renewal and tumorigenesis by reshaping methionine metabolism in triple-negative breast cancer

    Experiment Type

    Genomic

    Protocol

    Briefly, 107 to 108 TNBC cells (Control or EMSY) were harvested, crosslinking was performed with 1% formaldehyde or 1% paraformaldehyde for 10 minutes. To enhance cell lysis, we ran the lysate through a 27g needle three times and flash froze it in −80°C. Sonication was then performed with the Misonix 4000 water bath sonication unit at 15% amplitude for 20 minutes. Protein/DNA complex was precipitated by specific antibodies against H3K4me2/3 (CST) and IgG control (Millipore, Burlington, MA, USA). DNA was purified using DNA Purification Kit (Qiagen, Hilden, Germany). PCR was performed using Phusion High Fidelity PCR master mix. ChIP-enriched chromatins were sequenced on Illumina HiSeq 2000 platforms according to the manufacture’s protocol.

    OEX025490

  • EXPR PDAC_RNAseq

    Xinjing  Wang, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 2023.11.20

    Project ID

    OEP004748

    Project Name

    KRAS-G12V mRNA Vaccine and Pembrolizumab in Advanced Solid Tumors: Case report

    Experiment Type

    Genomic

    OEX025487

  • EXPR PDAC_TCR

    Xinjing  Wang, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 2023.11.20

    Project ID

    OEP004748

    Project Name

    KRAS-G12V mRNA Vaccine and Pembrolizumab in Advanced Solid Tumors: Case report

    Experiment Type

    Genomic

    OEX025488

  • EXPR PDAC_WES

    Xinjing  Wang, Ruijin Hospital, Shanghai Jiaotong University School of Medicine, 2023.11.20

    Project ID

    OEP004748

    Project Name

    KRAS-G12V mRNA Vaccine and Pembrolizumab in Advanced Solid Tumors: Case report

    Experiment Type

    Genomic

    OEX025486

  • EXPR OEX_Gu_2311171946

    GuLan  chun, , 2023.11.17

    Project ID

    OEP004747

    Project Name

    20231117_rawdata

    Experiment Type

    Genomic

    OEX025485

  • EXPR OEX_meiyao_2311161809

    meiyao  he, , 2023.11.16

    Project ID

    OEP004742

    Project Name

    Spatial transcriptomics analysis of tumor microenvironment in Epstein-Barr virus (EBV)-positive diffuse large B-cell lymphoma (DLBCL

    Experiment Type

    Transcriptomic

    OEX025480

  • EXPR Proteome

    cheng  cheng, Shanghai Jiaotong University School of Medicine affiliated Xinhua Hospital, 2023.11.08

    Project ID

    OEP004700

    Project Name

    Proteomic and phospho-proteomic raw data of Wilms' tumor tissue and normal adjacent kidney tissue.

    Experiment Type

    Proteomic

    Protocol

    TMT 6-plex shotgun proteomics was performed on Wilms' tumor tissue and normal adjacent kidney tissue from Wilms tumor patients using a Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific). Each experiment has two technical replications.

    OEX025368

  • EXPR Cell line RNA-seq

    cheng  cheng, Shanghai Jiaotong University School of Medicine affiliated Xinhua Hospital, 2023.11.10

    Project ID

    OEP004700

    Project Name

    Proteomic and phospho-proteomic raw data of Wilms' tumor tissue and normal adjacent kidney tissue.

    Experiment Type

    Genomic

    OEX025392

  • EXPR Phosphoproteome

    cheng  cheng, Shanghai Jiaotong University School of Medicine affiliated Xinhua Hospital, 2023.11.08

    Project ID

    OEP004700

    Project Name

    Proteomic and phospho-proteomic raw data of Wilms' tumor tissue and normal adjacent kidney tissue.

    Experiment Type

    Proteomic

    Protocol

    Label-free Phospho-proteomic was performed on Wilms' tumor tissue and normal adjacent kidney tissue from Wilms tumor patients using a Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific).

    OEX025369

  • EXPR Zhangnai Yoonia

    Xiaoyuan  Feng, , 2023.11.13

    Project ID

    OEP004731

    Project Name

    Genomics of lacustrine Roseobacteraceae isolates from Tibetan Plateau lakes

    Experiment Type

    Genomic

    Protocol

    Genomic DNA was extracted using the Wizard Genomic DNA Purification Kit (Promega) and subse-quently sequenced on the Illumina Miseq platform (Shanghai Biozeron Biotechnology Co., Ltd).

    OEX025396