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  • EXPR Gradient-dilution culturing_16S_information

    Meiying  Xu, Guangdong Institute of Microbiology, 2021.01.19

    Project ID

    OEP001305

    Project Name

    Lab enriched black odorous sediment microbial communites from Pearl River Delt, Guangzhou, China

    Experiment Type

    Metagenomic

    OEX012008

  • EXPR SB8_metagenomic

    Meiying  Xu, Guangdong Institute of Microbiology, 2021.01.15

    Project ID

    OEP001305

    Project Name

    Lab enriched black odorous sediment microbial communites from Pearl River Delt, Guangzhou, China

    Experiment Type

    Metagenomic

    OEX011865

  • EXPR SB8_16S

    Meiying  Xu, Guangdong Institute of Microbiology, 2021.03.10

    Project ID

    OEP001305

    Project Name

    Lab enriched black odorous sediment microbial communites from Pearl River Delt, Guangzhou, China

    Experiment Type

    Metagenomic

    OEX012493

  • EXPR OEX_Yuxiang_2401091954

    Yuxiang  Song, , 2024.01.09

    Project ID

    OEP004972

    Project Name

    Enrichment of soil ammonia-oxidizing organisms

    Experiment Type

    Genomic

    OEX027196

  • EXPR OEX_Jianjun_2309141602

    Jianjun  Wang, Nanjing Institute of Geography and Limnology, 2023.09.14

    Project ID

    OEP004545

    Project Name

    Microbial diversity in Chinese lake sediments

    Experiment Type

    Genomic

    OEX025163

  • EXPR Tidal effects on cable bacteria and phenanthrene biodegradation

    Meiying  Xu, , 2023.12.31

    Project ID

    OEP004935

    Project Name

    Tidal effects on cable bacteria and phenanthrene biodegradation

    Experiment Type

    Genomic

    OEX027126

  • EXPR m6A

    wenting  Xu, Zhejiang Chinese Medical University, 2024.01.04

    Project ID

    OEP004951

    Project Name

    m6A

    Experiment Type

    Genomic

    Protocol

    Total RNA was isolated and purified using TRIzol reagent (Invitrogen, Carlsbad, CA, USA) following the manufacturer s procedure. The RNA amount and purity of each sample was quantified using NanoDrop ND-1000 (NanoDrop, Wilmington, DE, USA), and the RNA integrity was assessed by Bioanalyzer 2100 (Agilent, CA, USA). Poly (A) RNA was purified from 50μg total RNA using Dynabeads Oligo (dT)25-61005 (Thermo Fisher, CA, USA) by two rounds of purification. Then the poly(A) RNA was fragmented into small pieces using Magnesium RNA Fragmentation Module (NEB, cat.e6150, USA) under 86℃ 7min. The cleaved RNA fragments were incubated for 2h at 4℃ with m6A-specific antibody (No. 202003, Synaptic Systems, Germany) in IP buffer (50 mM Tris-HCl, 750 mM NaCl and 0.5% Igepal CA-630). The IP RNA was reverse-transcribed to cDNA by SuperScript™ II Reverse Transcriptase (Invitrogen, cat. 1896649, USA), which was next used to synthesise U-labeled second-stranded DNAs with E. coli DNA polymerase I (NEB, cat.m0209, USA), RNase H (NEB, cat.m0297, USA) and dUTP Solution (Thermo Fisher, cat.R0133, USA). An A-base was added to the blunt ends of each strand, preparing for ligation to the indexed adapters. Dual-index adapters were ligated to the fragments, and size selection was performed with AMPureXP beads. After the heat-labile UDG enzyme (NEB, cat.m0280, USA) treatment of the U-labeled second-stranded DNAs, the ligated products were amplified with PCR by the following conditions: initial denaturation at 95℃ for 3 min; 8 cycles of denaturation at 98℃ for 15 sec, annealing at 60℃ for 15 sec, and extension at 72℃ for 30 sec; and then final extension at 72℃ for 5 min. At last, we performed paired-end sequencing (PE150) on an Illumina Novaseq™ 6000 platform(LC-Bio Technology CO., Ltd., Hangzhou, China) following the vendor s recommended protocol.

    OEX027149

  • EXPR Lipidomics of SOAT1 knockdown DU145 cells negative

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.12.29

    Project ID

    OEP004894

    Project Name

    Lipidomics of SOAT1 knockdown DU145 cells

    Experiment Type

    Metabolomic

    Protocol

    control and experimental samples are extracted using methanol containing internal standards and then lyophilized. The samples are subjected to LC-MS analysis.

    OEX027032

  • EXPR Lipidomics of SOAT1 knockdown DU145 cells positive

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.12.29

    Project ID

    OEP004894

    Project Name

    Lipidomics of SOAT1 knockdown DU145 cells

    Experiment Type

    Metabolomic

    Protocol

    control and experimental samples are extracted using methanol containing internal standards and then lyophilized. The samples are subjected to LC-MS analysis.

    OEX027031

  • EXPR Lipidomics of SOAT1 overexpressed DU145 cells negative

    Yuanyuan  Luo, Dalian institute of chemical physics, 2023.12.29

    Project ID

    OEP004895

    Project Name

    Lipidomics of SOAT1 overexpressed DU145 cells

    Experiment Type

    Metabolomic

    Protocol

    control and experimental samples are extracted using methanol containing internal standards and then lyophilized. The samples are subjected to LC-MS analysis.

    OEX027034