Jinxin  Xu, Xiamen University, 2023.03.08

Project ID

OEP003994

Project Name

The microbial communities in the coastal regions of China

Experiment Type

Genomic

OEX021445

Jinxin  Xu, Xiamen University, 2023.03.08

Project ID

OEP003994

Project Name

The microbial communities in the coastal regions of China

Experiment Type

Genomic

OEX021443

Jianxiang  Shi, Zhengzhou University, 2023.05.25

Project ID

OEP004164

Project Name

Identification of potential resistance mechanisms and therapeutic targets for the relapse of BCMA CAR-T therapy in relapsed/refractory multiple myeloma through single-cell sequencing

Experiment Type

Transcriptomic single cell

Protocol

Our group launched a single-arm, single-center clinical trial on BCMA CAR-T cells in the treatment of R/R-MM on April 11, 2018. The construction of anti-BCMA CAR, lentivirus production and production of CAR-T cells were described by our previous study [11]. Thirty-four patients with R/R-MM were treated with BCMA CAR-T cell therapy and the preliminary results have been reported in the journal of leukemia (ClinicalTrials.gov Identifier: NCT03661554) [11]. The ORR is 88.2% and the median progression-free survival time (PFS) is 12.1 months [11]. To analyze the mechanism of relapse after BCMA CAR-T cell therapy, one patient at baseline (R/R-MM) and one patient at progression (first relapse after BCMA CAR-T therapy) were enrolled in the study. The patient relapse after BCMA CAR-T cell therapy is from one of the 34 patients, who was enrolled in this clinical trial on December 13, 2019, and a relapse was detected on October 27, 2021(namely as progression in this study). And the other patient is from the newly enrolled, who met the criteria for enrollment in the clinical trial on December 3, 2021, after screening (namely as baseline in this study). The detailed characteristics of these two patients including clinicopathological features, bone marrow plasma cell ratio, staging and so on were comparable and similar (Additional file 1: Table S1). Five milliliters (mL) of bone marrow were then extracted from the two patients, and the CD45+ cells were enriched with human CD45 microbeads, according to the manufacturer’s instructions (Miltenyi Biotec, Cat#:130-045-801). The CD45+ cells were subsequently sent for 10 × genomics ScRNA-seq.

OEX023766

Jianmin  Zhang, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 2023.05.24

Project ID

OEP004162

Project Name

Serum metabolome of IIM: MALDI-TOF

Experiment Type

Metabolomic

OEX023764

Jianmin  Zhang, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College, 2023.05.25

Project ID

OEP004163

Project Name

Serum metabolome of IIM: LC-MS

Experiment Type

Metabolomic

OEX023765

Huanhuan  Wei, , 2023.05.23

Project ID

OEP004150

Project Name

The SLK Splicing Switch: A Promising Target for Cancer Metastasis Regulation

Experiment Type

Transcriptomic

OEX023758

Liting  Jiang, , 2023.05.15

Project ID

OEP004145

Project Name

Transcriptome-based network analysis related to Tfh cells in Primary Sjogren Syndrome

Experiment Type

Transcriptomic

OEX023216

Yun  Tian, Xiamen University, 2021.03.15

Project ID

OEP001343

Project Name

Microbial response of mangrove microbiome to BaP and nitrate contamination

Experiment Type

Metagenomic

Protocol

PCR amplification of 16S rRNA hypervariable region V3-V4

OEX012584

Jianxiang  Shi, Zhengzhou University, 2023.05.08

Project ID

OEP004136

Project Name

Bufalin Enhances Radiosensitivity of Esophageal Squamous Carcinoma Cells by Suppressing Homologous Recombination Repair Pathway

Experiment Type

Transcriptomic

Protocol

Total RNA was extracted from KYSE150 cells using Cell Total RNA Extraction Kit (TIANGEN; Beijing, China). RNA integrity was verified by Agilent 4200 Bioanalyzer (CA, USA) and RNA quantification was performed by ND-2000 (Nanodroplet technology). The MGIEasy-rRNA Removal kit (BGI; Shenzhen, China) was used to remove rRNA, and the library was prepared according to the MGIEasy-RNA Library Preparation kit (96RXN). The qualified cDNA library was sequenced by BGI high-throughput sequencing platform, and the original sequencing data were finally obtained.

OEX023215

Yu  Zhang, Shanghai Jiaotong University, 2023.02.23

Project ID

OEP003879

Project Name

MT-DeepSea

Experiment Type

Metatranscriptomic

Protocol

Ribo-Zero™ rRNA Removal Kit; NEBNext® Ultra IITM Directional RNA Library Prep Kit for Illumina® with Illumina adapters

OEX021341