General Information
- Project ID
- OEP000035
- Project Name
- Sp8 and Sp9 Coordinately Promote Striatal Medium Spiny Neuron Production through Activating Six3 Expression
- Description
- D1- and D2-type medium spiny neurons (MSNs) are the principal projection neurons in the striatum, including in the dorsal striatum (caudate nucleus and putamen) and ventral striatum (nucleus accumbens and olfactory tubercle) that are generated by the lateral ganglionic eminence (LGE). Using conditional deletion, we show that mice lacking the Sp8 and Sp9 transcription factors (TFs) selectively have a severe reduction in D2 MSNs due to reduced neurogenesis in the LGE. Sp8/9 together drive expression of the Six3 TF in a spatial restricted domain of the LGE subventricular zone. Conditional deletion of Six3 also prevents the formation of most D2 MSNs, phenocopying the Sp8/9 loss of function. Finally, ChIP-Seq reveals that SP9 directly binds to the promoter and a putative enhancer of Six3. This study provides evidence for components of a transcription pathway, in a regionally restricted LGE domain, that selectively drives the generation of D2 MSNs.
- Publications
-
SP8 and SP9 coordinately promote D2-type medium spiny neuron production by activating six3expression.
Xu, Z., Liang, Q., Song, X., Zhang, Z., Lindtner, S., & Li, Z., et al. (2018). Sp8 and sp9 coordinately promote d2-type medium spiny neuron production by activating six3 expression. Development, dev.165456.
(PMID: 29967281) (DOI: 10.1242/dev.165456)
Project information
- Experiments
- 2
- Samples
- 10
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES001106 Sp89-DCKO1A2 Animalia Mus musculus N/A 2018-05-24 OES001107 Sp89-DCKO2A3 Animalia Mus musculus N/A 2018-05-24 OES001108 Sp89-DCKO3A4 Animalia Mus musculus N/A 2018-05-24 OES001109 Control1A9 Animalia Mus musculus N/A 2018-05-24 OES001110 Control2A10 Animalia Mus musculus N/A 2018-05-24 OES001111 Control3B5 Animalia Mus musculus N/A 2018-05-24 OES001112 Sp9_E13_ChIP Animalia Mus musculus N/A 2018-05-24 OES001113 Sp9_E13_Input Animalia Mus musculus N/A 2018-05-24 OES001114 Sp9_E16_ChIP Animalia Mus musculus N/A 2018-05-24 OES001115 Sp9_E16_Input Animalia Mus musculus N/A 2018-05-24 - Runs
-
16
Run ID Run Name Experiment Sample Data Num Create Date OER004823 Run_Sp89-DCKO1A2 OEX000044 OES001106 1 2018-05-24 OER004824 Run_Sp89-DCKO1A2 OEX000044 OES001106 1 2018-05-24 OER004825 Run_Sp89-DCKO2A3 OEX000044 OES001107 1 2018-05-24 OER004826 Run_Sp89-DCKO2A3 OEX000044 OES001107 1 2018-05-24 OER004827 Run_Sp89-DCKO3A4 OEX000044 OES001108 1 2018-05-24 OER004828 Run_Sp89-DCKO3A4 OEX000044 OES001108 1 2018-05-24 OER004829 Run_Control1A9 OEX000044 OES001109 1 2018-05-24 OER004830 Run_Control1A9 OEX000044 OES001109 1 2018-05-24 OER004831 Run_Control2A10 OEX000044 OES001110 1 2018-05-24 OER004832 Run_Control2A10 OEX000044 OES001110 1 2018-05-24 OER004833 Run_Control3B5 OEX000044 OES001111 1 2018-05-24 OER004834 Run_Control3B5 OEX000044 OES001111 1 2018-05-24 OER004835 Run_Sp9_E13_ChIP OEX000045 OES001112 1 2018-05-24 OER004836 Run_Sp9_E13_Input OEX000045 OES001113 1 2018-05-24 OER004837 Run_Sp9_E16_ChIP OEX000045 OES001114 1 2018-05-24 OER004838 Run_Sp9_E16_Input OEX000045 OES001115 1 2018-05-24
Author Information
- Create Date
- 2018-05-24
- Last Modified
- 2022-05-07
- Submission
- hua feng
- PICB