General Information
- Project ID
- OEP000077
- Project Name
- An extensive program of periodic alternative splicing linked to cell cycle progression
- Description
- Progression through the mitotic cell cycle requires periodic regulation of gene function at the levels of transcription, translation, protein-protein interactions, post-translational modification and degradation. However, the role of alternative splicing (AS) in the temporal control of cell cycle is not well understood. By sequencing the human transcriptome through two continuous cell cycles, we identify ~1,300 genes with cell cycle-dependent AS changes. These genes are significantly enriched in functions linked to cell cycle control, yet they do not significantly overlap genes subject to periodic changes in steady-state transcript levels. Many of the periodically spliced genes are controlled by the SR protein kinase CLK1, whose level undergoes cell cycle-dependent fluctuations via an auto-inhibitory circuit. Disruption of CLK1 causes pleiotropic cell cycle defects and loss of proliferation, whereas CLK1 over-expression is associated with various cancers. These results thus reveal a large program of CLK1-regulated periodic AS intimately associated with cell cycle control.
- Used ID
- NODEP00000042  
- Publications
-
An extensive program of periodic alternative splicing linked to cell cycle progression
Dominguez D, Tsai Y H, Weatheritt R, et al. An extensive program of periodic alternative splicing linked to cell cycle progression[J]. Elife, 2016, 5.
(PMID: 27015110) (DOI: 10.7554/eLife.10288)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX000085 ZFWang_lab_NODEX00000042 Transcriptomic 2016-05-20 - Samples
- 14
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES002003 D1 Cell line Homo sapiens HeLa cells 2016-05-20 OES002004 D3 Cell line Homo sapiens HeLa cells 2016-05-20 OES002005 D2 Cell line Homo sapiens HeLa cells 2016-05-20 OES002006 D4A Cell line Homo sapiens HeLa cells 2016-05-20 OES002007 D5 Cell line Homo sapiens HeLa cells 2016-05-20 OES002008 D6 Cell line Homo sapiens HeLa cells 2016-05-20 OES002009 D7 Cell line Homo sapiens HeLa cells 2016-05-20 OES002010 D8 Cell line Homo sapiens HeLa cells 2016-05-20 OES002011 D9 Cell line Homo sapiens HeLa cells 2016-05-20 OES002012 D10 Cell line Homo sapiens HeLa cells 2016-05-20 OES002013 D11 Cell line Homo sapiens HeLa cells 2016-05-20 OES002014 D12 Cell line Homo sapiens HeLa cells 2016-05-20 OES002015 D13 Cell line Homo sapiens HeLa cells 2016-05-20 OES002016 D14 Cell line Homo sapiens HeLa cells 2016-05-20 - Runs
-
14
Run ID Run Name Experiment Sample Data Num Create Date OER008964 ZFWang_lab_NODER00000395 OEX000085 OES002007 2 2016-05-20 OER008965 ZFWang_lab_NODER00000396 OEX000085 OES002015 2 2016-05-20 OER008966 ZFWang_lab_NODER00000397 OEX000085 OES002003 2 2016-05-20 OER008967 ZFWang_lab_NODER00000398 OEX000085 OES002004 2 2016-05-20 OER008968 ZFWang_lab_NODER00000399 OEX000085 OES002011 2 2016-05-20 OER008969 ZFWang_lab_NODER00000400 OEX000085 OES002016 2 2016-05-20 OER008970 ZFWang_lab_NODER00000401 OEX000085 OES002014 2 2016-05-20 OER008971 ZFWang_lab_NODER00000402 OEX000085 OES002012 2 2016-05-20 OER008972 ZFWang_lab_NODER00000403 OEX000085 OES002013 2 2016-05-20 OER008973 ZFWang_lab_NODER00000404 OEX000085 OES002008 2 2016-05-20 OER008974 ZFWang_lab_NODER00000405 OEX000085 OES002009 2 2016-05-20 OER008975 ZFWang_lab_NODER00000406 OEX000085 OES002006 2 2016-05-20 OER008976 ZFWang_lab_NODER00000407 OEX000085 OES002005 2 2016-05-20 OER008977 ZFWang_lab_NODER00000408 OEX000085 OES002010 2 2016-05-20
Author Information
- Create Date
- 2016-05-20
- Last Modified
- 2018-12-26
- Submission
- Xiaojuan Fan
- PICB