General Information
- Project ID
- OEP000093
- Project Name
- Graphene Sheet-Induced Global Maturation of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells
- Description
- Abstract: Human induced pluripotent stem cells (hiPSCs) can proliferate infinitely. Their ability to differentiate into cardiomyocytes provides abundant sources for disease modeling, drug screening and regenerative medicine. However, hiPSC-derived cardiomyocytes (hiPSC-CMs) display a low degree of maturation and fetal-like properties. Current in vitro differentiation methods do not mimic the structural, mechanical, and physiological properties of the cardiogenesis niche. Recently, we present an efficient cardiac maturation platform that combines hiPSCs monolayer cardiac differentiation with graphene substrate which is a biocompatible and superconductive material. The hiPSCs lines were successfully maintained on the graphene sheets, and were able to differentiate into functional cardiomyocytes. This strategy markedly increased the myofibril ultrastructural organization, elevated the conduction velocity and enhanced both the Ca2+ handling and electrophysiological properties in the absence of electrical stimulation. On the graphene substrate, the expression of connexin 43 increased along with the conduction velocity. Interestingly, the Bone morphogenetic proteins signaling was also significantly activated during early cardiogenesis, confirmed by RNA sequencing analysis. Here, we reasoned that graphene substrate as a conductive biomimetic surface could facilitate the intrinsic electrical propagation, mimicking the microenvironment of the native heart, to further promote the global maturation of hiPSC-CMs. Our findings highlight the capability of electrically active substrates to influence cardiomyocyte development. We believe that application of graphene sheets will be useful for simple, fast and scalable maturation of regenerated cardiomyocytes.
- Used ID
- NODEP00000217  
- Publications
-
Graphene Sheet-Induced Global Maturation of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells
Wang J, Cui C, Nan H, et al. Graphene Sheet-Induced Global Maturation of Cardiomyocytes Derived from Human Induced Pluripotent Stem Cells[J]. Acs Applied Materials & Interfaces, 2017, 9(31):25929.
(PMID: 28718622) (DOI: 10.1021/acsami.7b08777)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX000093 Fenghua_NODEX1705081001 Transcriptomic 2017-05-08 - Samples
- 6
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES002274 C1 Human Homo sapiens blood 2017-05-08 OES002275 C2 Human Homo sapiens blood 2017-05-08 OES002276 C3 Human Homo sapiens blood 2017-05-08 OES002277 G1 Human Homo sapiens blood 2017-05-08 OES002278 G2 Human Homo sapiens blood 2017-05-08 OES002279 G3 Human Homo sapiens blood 2017-05-08 - Runs
-
6
Run ID Run Name Experiment Sample Data Num Create Date OER009031 Fenghua_NODER1705081042 OEX000093 OES002274 2 2017-05-08 OER009032 Fenghua_NODER1705081044 OEX000093 OES002275 2 2017-05-08 OER009033 Fenghua_NODER1705081046 OEX000093 OES002276 2 2017-05-08 OER009034 Fenghua_NODER1705081048 OEX000093 OES002277 2 2017-05-08 OER009035 Fenghua_NODER1705081049 OEX000093 OES002278 2 2017-05-08 OER009036 Fenghua_NODER1705081050 OEX000093 OES002279 2 2017-05-08
Author Information
- Create Date
- 2017-05-08
- Last Modified
- 2018-12-26
- Submission
- hua feng
- PICB