General Information
- Project ID
- OEP000103
- Project Name
- Retinoblastoma samples RNA-seq
- Description
- Retinoblastoma(3 tumor versus 3 para-tumor samples) RNA-sequencing Aberrant chromatin transformation dysregulates gene expression and may be an important driver of tumorigenesis. However, the functional role of chromosomal dynamics in tumorigenesis remains to be elucidated. Here, using in vitro and in vivo experiments, we reveal a novel long noncoding (lncing) driver at chr12p13.3, in which a novel lncRNA GALNT8 Antisense Upstream 1 (GAU1) is initially activated by an open chromatin status, triggering recruitment of the transcription elongation factor TCEA1 at the oncogene GALNT8 promoter and cis-activates the expression of GALNT8. Analysis of The Cancer Genome Atlas (TCGA) clinical database revealed that the GAU1/GALNT8 driver serves as an important indicative biomarker, and targeted silencing of GAU1 via the HKP-encapsulated method exhibited therapeutic efficacy in orthotopic xenografts. Our study presents a novel oncogenetic mechanism in which aberrant tuning of the chromatin state at specific chromosomal loci exposes factor-binding sites, leading to recruitment of trans-factor and activation of oncogenetic driver, thereby provide a novel alternative concept of chromatin dynamics in tumorigenesis.
- Used ID
- NODEP0037193   NODEP00371793  
- Publications
-
Dynamic chromosomal tuning of a novel GAU1lncing driver at chr12p13.32 accelerates tumorigenesis
Chai P , Jia R , Jia R , et al. Dynamic chromosomal tuning of a novel GAU1 lncing driver at chr12p13.32 accelerates tumorigenesis[J]. Nucleic Acids Research, 2018, 46(12):52.
(PMID: 29741668) (DOI: 10.1093/nar/gky366)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX000106 retinoblastoma sequencing Transcriptomic 2018-02-24 - Samples
- 6
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES002923 Retinoblastoma-1 Human Homo sapiens N/A 2018-02-24 OES002924 Retinoblastoma-2 Human Homo sapiens N/A 2018-02-24 OES002925 Retinoblastoma-3 Human Homo sapiens N/A 2018-02-24 OES002926 Para retinoblastoma normal control-1 Human Homo sapiens N/A 2018-02-24 OES002927 Para retinoblastoma normal control-2 Human Homo sapiens N/A 2018-02-24 OES002928 Para retinoblastoma normal control-3 Human Homo sapiens N/A 2018-02-24 - Runs
-
7
Run ID Run Name Experiment Sample Data Num Create Date OER009389 NODER_Peiwei_1802241429 OEX000106 OES002926 1 2018-02-24 OER009390 NODER_Peiwei_1802241430 OEX000106 OES002926 1 2018-02-24 OER009391 NODER_Peiwei_1802241430 OEX000106 OES002927 2 2018-02-24 OER009392 NODER_Peiwei_1802241430 OEX000106 OES002928 2 2018-02-24 OER009393 NODER_Peiwei_1802241431 OEX000106 OES002923 2 2018-02-24 OER009394 NODER_Peiwei_1802241431 OEX000106 OES002924 2 2018-02-24 OER009395 NODER_Peiwei_1802241431 OEX000106 OES002925 2 2018-02-24 - Analysis
-
1
Analysis ID Analysis Title Analysis Type Data Date OEZ000380 NODEZ_Peiwei_1802241432 Sequence Annotation 2 2018-02-24
Author Information
- Create Date
- 2018-02-24
- Last Modified
- 2021-06-11
- Submission
- Peiwei Chai
- Shanghai Ninth people's hospital