General Information
- Project ID
- OEP000104
- Project Name
- Creating a functional single-chromosome yeast
- Description
- Eukaryotic genomes are generally organized in multiple chromosomes. Here we have created a functional single-chromosome yeast from a Saccharomyces cerevisiae haploid cell containing sixteen linear chromosomes, by successive end-to-end chromosome fusions and centromere deletions. The fusion of sixteen native linear chromosomes into a single chromosome results in marked changes to the global three-dimensional structure of the chromosome due to the loss of all centromere-associated inter-chromosomal interactions, most telomere-associated inter-chromosomal interactions and 67.4% of intra-chromosomal interactions. However, the single-chromosome and wild-type yeast cells have nearly identical transcriptome and similar phenome profiles. The giant single chromosome can support cell life, although this strain shows reduced growth across environments, competitiveness, gamete production and viability. This synthetic biology study demonstrates an approach to exploration of eukaryote evolution with respect to chromosome structure and function.
- Used ID
- NODEP00371807  
- Publications
-
Creating a functional single-chromosome yeast
Shao, Y., Lu, N., Wu, Z., Cai, C., Wang, S., & Zhang, L. L., et al. (2018). Creating a functional single-chromosome yeast. Nature.
(PMID: 30069045) (DOI: 10.1038/s41586-018-0382-x)
Project information
- Experiments
- 4
- Samples
- 5
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES000008 SY13 Microbe Saccharomyces cerevisiae N/A 2018-04-24 OES000009 SY6 Microbe Saccharomyces cerevisiae N/A 2018-04-24 OES002929 WT Microbe Saccharomyces cerevisiae N/A 2018-03-11 OES002930 SY14 Microbe Saccharomyces cerevisiae N/A 2018-03-11 OES013413 SY15 Microbe Saccharomyces cerevisiae N/A 2018-10-30 - Runs
-
13
Run ID Run Name Experiment Sample Data Num Create Date OER000040 SY14 HiC sequencing OEX000016 OES002930 4 2018-04-24 OER000133 SY13 HiC_sequencing OEX000016 OES000008 4 2018-04-27 OER000134 SY6_genome_HiC_sequencing OEX000016 OES000009 4 2018-04-27 OER022851 Wild Type HiC OEX000016 OES002929 4 2018-07-22 OER024570 SY15 HiC sequencing OEX000016 OES013413 4 2018-10-30 OER000135 WT_transcriptome_sequencing OEX000017 OES002929 6 2018-04-27 OER022078 SY14 Transcriptome Sequencing OEX000017 OES002930 6 2018-07-16 OER024568 SY14_Trancriptome batch 2 OEX000017 OES002930 6 2018-10-30 OER024569 SY15 Transcriptome OEX000017 OES013413 6 2018-10-30 OER009396 WT first cell and second cell genome assembly results OEX000107 OES002929 2 2018-03-11 OER000136 WT NGS genome OEX000107 OES002929 6 2018-04-27 OER009397 SY14 genome assembly results OEX000108 OES002930 1 2018-03-11 OER000041 SY14 NGS sequencing OEX000108 OES002930 2 2018-04-24
Author Information
- Create Date
- 2018-03-11
- Last Modified
- 2019-08-21
- Submission
- Xiaoli Xue
- Institute of Plant Physiology and Ecolog