General Information
- Project ID
- OEP000114
- Project Name
- BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity.
- Description
- Base editor (BE), containing a cytidine deaminase and catalytically defective Cas9, has been widely used to perform base editing. However, the narrow editing window of BE limits its utility. Here, we developed a new editing technology named as base editor for programming larger C to U (T) scope (BE-PLUS) by fusing 10 copies of GCN4 peptide to nCas9(D10A) for recruiting scFv-APOBEC-UGI-GB1 to the target sites. The new system achieves base editing with a broadened window, resulting in an increased genome-targeting scope. Interestingly, the new system yielded much fewer unwanted indels and non-C-to-T conversions. We also demonstrated its potential use in gene disruption across the whole genome through induction of stop codons (iSTOP). Taken together, the BE-PLUS system offers a new editing tool with increased editing window and enhanced fidelity.
- Used ID
- NODEP00371730  
- Publications
-
BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
Jiang W , Feng S, Huang S , Yu W , Li G, Yang G , Liu Y ,et al. (2018). BE-PLUS: a new base editing tool with broadened editing window and enhanced fidelity
(PMID: 29875396) (DOI: 10.1038/s41422-018-0052-4)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX001432 The base editor for programming larger C to U (T) scope (BE-PLUS) Genomic 2017-12-12 - Samples
- 7
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES004574 NODES_GCN4-D10A-1 Human Homo sapiens N/A 2017-12-12 OES004575 NODES_GCN4-D10A-2 Human Homo sapiens N/A 2017-12-12 OES004576 NODES_GCN4-D10A-3 Human Homo sapiens N/A 2017-12-12 OES004577 NODES_BE3-1 Human Homo sapiens N/A 2017-12-12 OES004578 NODES_BE3-2 Human Homo sapiens N/A 2017-12-12 OES004579 NODES_BE3-3 Human Homo sapiens N/A 2017-12-12 OES004580 NODES_CTRL Human Homo sapiens N/A 2017-12-12 - Runs
-
7
Run ID Run Name Experiment Sample Data Num Create Date OER010055 NODER_GCN4-D10A-1 OEX001432 OES004574 2 2017-12-12 OER010056 NODER_GCN4-D10A-2 OEX001432 OES004575 2 2017-12-12 OER010057 NODER_GCN4-D10A-3 OEX001432 OES004576 2 2017-12-12 OER010058 NODER_BE3-1 OEX001432 OES004577 2 2017-12-12 OER010059 NODER_BE3-2 OEX001432 OES004578 2 2017-12-12 OER010060 NODER_BE3-3 OEX001432 OES004579 2 2017-12-12 OER010061 NODER_CTRL OEX001432 OES004580 2 2017-12-12
Author Information
- Create Date
- 2017-12-12
- Last Modified
- 2019-08-21
- Submission
- Wen Jiang
- ShanghaiTech University