General Information
- Project ID
- OEP000195
- Project Name
- Cytosine base editor generates substantial off-target single nucleotide variants in mouse embryos
- Description
- Genome editing holds promise for correcting pathogenic mutations. However, it is difficult to determine off-target effects of editing due to single-nucleotide polymorphism in individuals. Here we developed a method named GOTI (genome-wide off-target analysis by two-cell embryo injection) to detect off-target mutations by editing one blastomere of two-cell mouse embryos using either CRISPR-Cas9 or base editors. Comparison of the whole-genome sequences of progeny cells of edited and nonedited blastomeres at embryonic day 14.5 showed that off-target single-nucleotide variants (SNVs) were rare in embryos edited by CRISPR-Cas9 or adenine base editor, with a frequency close to the spontaneous mutation rate. By contrast, cytosine base editing induced SNVs at more than 20-fold higher frequencies, requiring a solution to address its fidelity.
- Publications
-
Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos
Erwei, Z., Yidi, S., Wu, W., Tanglong, Y., Wenqin, Y., & Hao, S., et al. (2018). Cytosine base editor generates substantial off-target single-nucleotide variants in mouse embryos. Science.
(PMID: 30819928) (DOI: 10.1126/science.aav9973)
Project information
- Experiments
- 2
- Samples
- 50
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES013948 B15 Animalia Mus musculus N/A 2018-11-30 OES013949 B16 Animalia Mus musculus N/A 2018-11-30 OES013950 SRS4025808 Animalia Mus musculus N/A 2018-11-30 OES013951 SRS4025809 Animalia Mus musculus N/A 2018-11-30 OES013952 SRS4025806 Animalia Mus musculus N/A 2018-11-30 OES013953 SRS4025807 Animalia Mus musculus N/A 2018-11-30 OES013954 B23 Animalia Mus musculus N/A 2018-11-30 OES013955 B24 Animalia Mus musculus N/A 2018-11-30 OES013956 B25 Animalia Mus musculus N/A 2018-11-30 OES013957 B26 Animalia Mus musculus N/A 2018-11-30 OES013958 B29 Animalia Mus musculus N/A 2018-11-30 OES013959 B30 Animalia Mus musculus N/A 2018-11-30 OES013960 B33 Animalia Mus musculus N/A 2018-11-30 OES013961 B34 Animalia Mus musculus N/A 2018-11-30 OES013962 SRS4025812 Animalia Mus musculus N/A 2018-11-30 OES013963 SRS4025813 Animalia Mus musculus N/A 2018-11-30 OES013964 SRS4025810 Animalia Mus musculus N/A 2018-11-30 OES013965 SRS4025811 Animalia Mus musculus N/A 2018-11-30 OES013966 SRS4025814 Animalia Mus musculus N/A 2018-11-30 OES013967 SRS4025815 Animalia Mus musculus N/A 2018-11-30 OES013968 SRS4025804 Animalia Mus musculus N/A 2018-11-30 OES013969 SRS4025805 Animalia Mus musculus N/A 2018-11-30 OES013970 SRS2549038 Animalia Mus musculus N/A 2018-11-30 OES013971 SRS2549039 Animalia Mus musculus N/A 2018-11-30 OES013972 SRS2549032 Animalia Mus musculus N/A 2018-11-30 OES013973 SRS2549035 Animalia Mus musculus N/A 2018-11-30 OES013974 SRS2549042 Animalia Mus musculus N/A 2018-11-30 OES013975 SRS2549043 Animalia Mus musculus N/A 2018-11-30 OES013976 SRS2549029 Animalia Mus musculus N/A 2018-11-30 OES013977 SRS2549030 Animalia Mus musculus N/A 2018-11-30 OES013978 SRS2549033 Animalia Mus musculus N/A 2018-11-30 OES013979 SRS2549034 Animalia Mus musculus N/A 2018-11-30 OES013980 SRS2549037 Animalia Mus musculus N/A 2018-11-30 OES013981 SRS2549036 Animalia Mus musculus N/A 2018-11-30 OES013982 SRS2549041 Animalia Mus musculus N/A 2018-11-30 OES013983 SRS2549028 Animalia Mus musculus N/A 2018-11-30 OES013984 SRS2604284 Animalia Mus musculus N/A 2018-11-30 OES013985 SRS2604285 Animalia Mus musculus N/A 2018-11-30 OES013986 SRS2604286 Animalia Mus musculus N/A 2018-11-30 OES013987 SRS2604287 Animalia Mus musculus N/A 2018-11-30 OES013988 SRS3024198 Animalia Mus musculus N/A 2018-11-30 OES013989 SRS3024199 Animalia Mus musculus N/A 2018-11-30 OES013990 SRS3024196 Animalia Mus musculus N/A 2018-11-30 OES013991 SRS3024197 Animalia Mus musculus N/A 2018-11-30 OES013992 SRS2549040 Animalia Mus musculus N/A 2018-11-30 OES013993 SRS2549031 Animalia Mus musculus N/A 2018-11-30 OES013994 CHG031191 Animalia Mus musculus N/A 2018-11-30 OES013995 CHG031192 Animalia Mus musculus N/A 2018-11-30 OES013996 CHG031193 Animalia Mus musculus N/A 2018-11-30 OES013997 CHG031194 Animalia Mus musculus N/A 2018-11-30 - Runs
-
50
Run ID Run Name Experiment Sample Data Num Create Date OER025228 B15 OEX001547 OES013948 2 2018-11-30 OER025229 B16 OEX001547 OES013949 2 2018-11-30 OER025230 SRR8169137 OEX001547 OES013950 2 2018-11-30 OER025231 SRR8169136 OEX001547 OES013951 2 2018-11-30 OER025232 SRR8169139 OEX001547 OES013952 2 2018-11-30 OER025233 SRR8169138 OEX001547 OES013953 2 2018-11-30 OER025234 B23 OEX001547 OES013954 2 2018-11-30 OER025235 B24 OEX001547 OES013955 2 2018-11-30 OER025236 B25 OEX001547 OES013956 2 2018-11-30 OER025237 B26 OEX001547 OES013957 2 2018-11-30 OER025238 B29 OEX001547 OES013958 2 2018-11-30 OER025239 B30 OEX001547 OES013959 2 2018-11-30 OER025240 B33 OEX001547 OES013960 2 2018-11-30 OER025241 B34 OEX001547 OES013961 2 2018-11-30 OER025242 SRR8169133 OEX001547 OES013962 2 2018-11-30 OER025243 SRR8169132 OEX001547 OES013963 2 2018-11-30 OER025244 SRR8169135 OEX001547 OES013964 2 2018-11-30 OER025245 SRR8169134 OEX001547 OES013965 2 2018-11-30 OER025246 SRR8169131 OEX001547 OES013966 2 2018-11-30 OER025247 SRR8169130 OEX001547 OES013967 2 2018-11-30 OER025248 SRR8169141 OEX001547 OES013968 2 2018-11-30 OER025249 SRR8169140 OEX001547 OES013969 2 2018-11-30 OER025250 SRR6109558 OEX001548 OES013970 2 2018-11-30 OER025251 SRR6109557 OEX001548 OES013971 2 2018-11-30 OER025252 SRR6109564 OEX001548 OES013972 2 2018-11-30 OER025253 SRR6109561 OEX001548 OES013973 2 2018-11-30 OER025254 SRR6109554 OEX001548 OES013974 2 2018-11-30 OER025255 SRR6109553 OEX001548 OES013975 2 2018-11-30 OER025256 SRR6109567 OEX001548 OES013976 2 2018-11-30 OER025257 SRR6109566 OEX001548 OES013977 2 2018-11-30 OER025258 SRR6109563 OEX001548 OES013978 2 2018-11-30 OER025259 SRR6109562 OEX001548 OES013979 2 2018-11-30 OER025260 SRR6109560 OEX001548 OES013980 2 2018-11-30 OER025261 SRR6109559 OEX001548 OES013981 2 2018-11-30 OER025262 SRR6109569 OEX001548 OES013982 2 2018-11-30 OER025263 SRR6109568 OEX001548 OES013983 2 2018-11-30 OER025264 SRR6185435 OEX001548 OES013984 2 2018-11-30 OER025265 SRR6185434 OEX001548 OES013985 2 2018-11-30 OER025266 SRR6185433 OEX001548 OES013986 2 2018-11-30 OER025267 SRR6185432 OEX001548 OES013987 2 2018-11-30 OER025268 SRR6812665 OEX001548 OES013988 2 2018-11-30 OER025269 SRR6812664 OEX001548 OES013989 2 2018-11-30 OER025270 SRR6812667 OEX001548 OES013990 2 2018-11-30 OER025271 SRR6812666 OEX001548 OES013991 2 2018-11-30 OER025272 SRR6109556 OEX001548 OES013992 2 2018-11-30 OER025273 SRR6109565 OEX001548 OES013993 2 2018-11-30 OER025274 CHG031191 OEX001548 OES013994 2 2018-11-30 OER025275 CHG031192 OEX001548 OES013995 2 2018-11-30 OER025276 CHG031193 OEX001548 OES013996 2 2018-11-30 OER025277 CHG031194 OEX001548 OES013997 2 2018-11-30 - Analysis
-
54
Analysis ID Analysis Title Analysis Type Data Date OEZ000416 B15 Other 1 2018-12-19 OEZ000417 B16 Other 1 2018-12-19 OEZ000418 B17 Other 1 2018-12-19 OEZ000419 B18 Other 1 2018-12-19 OEZ000420 B19 Other 1 2018-12-19 OEZ000421 B20 Other 1 2018-12-19 OEZ000422 B23 Other 1 2018-12-19 OEZ000423 B24 Other 1 2018-12-19 OEZ000424 B25 Other 1 2018-12-19 OEZ000425 B26 Other 1 2018-12-19 OEZ000426 B29 Other 1 2018-12-19 OEZ000427 B30 Other 1 2018-12-19 OEZ000428 B33 Other 1 2018-12-19 OEZ000429 B34 Other 1 2018-12-19 OEZ000430 CHG024597 Other 2 2018-12-19 OEZ000431 CHG024598 Other 2 2018-12-19 OEZ000432 CHG024599 Other 2 2018-12-19 OEZ000433 CHG024600 Other 2 2018-12-19 OEZ000434 CHG024601 Other 2 2018-12-19 OEZ000435 CHG024602 Other 2 2018-12-19 OEZ000436 CHG024603 Other 2 2018-12-19 OEZ000437 CHG024604 Other 2 2018-12-19 OEZ000438 CHG024605 Other 2 2018-12-19 OEZ000439 CHG024606 Other 2 2018-12-19 OEZ000440 CHG024607 Other 2 2018-12-19 OEZ000441 CHG024608 Other 2 2018-12-19 OEZ000442 CHG024609 Other 2 2018-12-19 OEZ000443 CHG024610 Other 2 2018-12-19 OEZ000444 CHG026384 Other 2 2018-12-19 OEZ000445 CHG026385 Other 2 2018-12-19 OEZ000446 CHG026386 Other 2 2018-12-19 OEZ000447 CHG026387 Other 2 2018-12-19 OEZ000448 CHG030181 Other 2 2018-12-19 OEZ000449 CHG030182 Other 2 2018-12-19 OEZ000450 CHG030183 Other 2 2018-12-19 OEZ000451 CHG030184 Other 2 2018-12-19 OEZ000452 CHG1708301 Other 2 2018-12-19 OEZ000453 CHG1708302 Other 2 2018-12-19 OEZ000454 s180904001WL Other 1 2018-12-19 OEZ000455 s180904002WL Other 1 2018-12-19 OEZ000456 s180904003WL Other 1 2018-12-19 OEZ000457 s180904004WL Other 1 2018-12-19 OEZ000458 s180904005WL Other 1 2018-12-19 OEZ000459 s180904006WL Other 1 2018-12-19 OEZ000460 s180904007WL Other 1 2018-12-19 OEZ000461 s180904008WL Other 1 2018-12-19 OEZ004387 SRR8599737 Other 1 2019-03-25 OEZ004388 SRR8599738 Other 1 2019-03-25 OEZ004389 SRR8599735 Other 1 2019-03-25 OEZ004390 SRR8599736 Other 1 2019-03-25 OEZ004391 SRR8599741 Other 1 2019-03-25 OEZ004392 SRR8599739 Other 1 2019-03-25 OEZ004393 SRR8599742 Other 1 2019-03-25 OEZ004411 A2 De Novo Assembly 1 2019-03-26
Author Information
- Create Date
- 2018-11-30
- Last Modified
- 2019-08-21
- Submission
- Hui Yang
- Institute of Neuroscience, State Key Laboratory of Neuroscience