General Information
- Project ID
- OEP001473
- Project Name
- Glycine deprivation induces DNA damage and thereby results in cell cycle arrest in myeloma cells
- Description
- Exogenous glycine deprivation inhibits tumor progression in multiple myeloma (MM) both in vitro and in vivo. To explore the underlying mechanisms of glycine metabolisms in MM cells, RNA-sequencing was performed in ARP1 cells cultured in the absence or presence of glycine (10 mg/L) for 24 hours. Genes involving glycine metabolism, among which SLC6A9, GLDC, SHMT2, and GSS were significantly highly expressed. This finding indicated that exogenous glycine deprivation mainly affected glycine transport, glycine cleavage, the interconversion between glycine and serine, and glutathione synthesis. In addition, enrichment analysis of signaling pathways based on different genes showed half of top 10 enriched pathways were related to cell mitosis and DNA damage.
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX011295 Glycine metabolism RNA-seq Transcriptomic 2021-01-08 - Samples
- 14
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES060195 Glycine_0mg_6hA1 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060196 Glycine_0mg_6h_A2 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060197 Glycine_0mg_12h_A1 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060198 Glycine_0mg_12h_A2 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060199 Glycine_0mg_24h_A1 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060200 Glycine_0mg_24h_A2 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060201 Glycine_10mg_6h_A1 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060202 Glycine_10mg_6h_A2 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060203 Glycine_10mg_12h_A1 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060204 Glycine_10mg_12h_A2 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060205 Glycine_10mg_24h_A1 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060206 Glycine_10mg_24h_A2 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060207 Normal_0h_A1 Cell line Homo sapiens BoneMarrow 2021-01-09 OES060208 Normal_0h_A2 Cell line Homo sapiens BoneMarrow 2021-01-09 - Runs
-
14
Run ID Run Name Experiment Sample Data Num Create Date OER094489 Glycine 0mg 6h A1 OEX011295 OES060195 2 2021-01-09 OER094490 Glycine 0mg 6h A2 OEX011295 OES060196 2 2021-01-09 OER094491 Glycine 0mg 12h A1 OEX011295 OES060197 2 2021-01-09 OER094492 Glycine 0mg 12h A2 OEX011295 OES060198 2 2021-01-09 OER094493 Glycine 0mg 24h A1 OEX011295 OES060199 2 2021-01-09 OER094494 Glycine 0mg 24h A2 OEX011295 OES060200 2 2021-01-09 OER094495 Glycine 10mg 6h A1 OEX011295 OES060201 2 2021-01-09 OER094496 Glycine 10mg 6h A2 OEX011295 OES060202 2 2021-01-09 OER094497 Glycine 10mg 12h A1 OEX011295 OES060203 2 2021-01-09 OER094498 Glycine 10mg 12h A2 OEX011295 OES060204 2 2021-01-09 OER094499 Glycine 10mg 24h A1 OEX011295 OES060205 2 2021-01-09 OER094500 Glycine 10mg 24h A2 OEX011295 OES060206 2 2021-01-09 OER094501 Normal 0h A1 OEX011295 OES060207 2 2021-01-09 OER094502 Normal 0h A2 OEX011295 OES060208 2 2021-01-09
Author Information
- Create Date
- 2021-01-08
- Last Modified
- 2023-12-01
- Submission
- Zhenhao Liu
- Central South University