General Information
- Project ID
- OEP001756
- Project Name
- Spatiotemporal Immune Landscape of Colorectal Cancer Liver Metastasis at Single-Cell Level
- Description
- Liver metastasis, the leading cause of colorectal cancer mortality, exhibits a highly heterogeneous and suppressive immune microenvironment. Here, we sequenced 97 matched samples by using single-cell RNA-seq and Spatial Transcriptomics. Strikingly, metastatic microenvironment underwent remarkable spatial reprogramming of immunosuppressive cells such as MRC1+ CCL18+ M2-like macrophages. We further developed scMetabolism, a computational pipeline for quantifying single-cell metabolism, and observed that those macrophages harbored enhanced metabolic activity. Interestingly, neoadjuvant chemotherapy could block this status and restore the antitumor immune balance in responsive patients, while the non-responsive patients deteriorated into a more suppressive one. Our work described the immune evolution of metastasis and uncovered the black box of how tumors respond to neoadjuvant chemotherapy.
- Publications
-
Spatiotemporal Immune Landscape of Colorectal Cancer Liver Metastasis at Single-Cell Level
Wu Y, Yang S, Ma J, et al. Spatiotemporal Immune Landscape of Colorectal Cancer Liver Metastasis at Single-Cell Level [published online ahead of print, 2021 Aug 20]. Cancer Discov. 2021;candisc.0316.2021. doi:10.1158/2159-8290.CD-21-0316
(PMID: 34417225) (DOI: 10.1158/2159-8290)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX012187 CRLM (scRNA-seq)-1 Transcriptomic 2021-01-29 - Samples
- 12
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES068438 CRLM (scRNA-seq)-P1 Human Homo sapiens CRLM 2021-01-29 OES068739 CRLM (scRNA-seq)-P2 Human Homo sapiens CRLM 2021-01-29 OES068740 CRLM (scRNA-seq)-P3 Human Homo sapiens CRLM 2021-01-29 OES068741 CRLM (scRNA-seq)-P4 Human Homo sapiens CRLM 2021-01-29 OES068742 CRLM (scRNA-seq)-P5 Human Homo sapiens CRLM 2021-01-29 OES068743 CRLM (scRNA-seq)-P6 Human Homo sapiens CRLM 2021-01-29 OES068744 CRLM (scRNA-seq)-P7 Human Homo sapiens CRLM 2021-01-29 OES068745 CRLM (scRNA-seq)-P8 Human Homo sapiens CRLM 2021-01-29 OES068746 CRLM (scRNA-seq)-P9 Human Homo sapiens CRLM 2021-01-29 OES068747 CRLM (scRNA-seq)-P10 Human Homo sapiens CRLM 2021-01-29 OES068748 CRLM (scRNA-seq)-P11 Human Homo sapiens CRLM 2021-01-29 OES068749 CRLM (scRNA-seq)-P12 Human Homo sapiens CRLM 2021-01-29 - Runs
-
12
Run ID Run Name Experiment Sample Data Num Create Date OER102694 OER_Yingcheng_2101292109_P01 OEX012187 OES068438 54 2021-01-29 OER102705 OER_Yingcheng_2101292146_P02 OEX012187 OES068739 24 2021-01-29 OER102706 OER_Yingcheng_2101292149_P03 OEX012187 OES068740 24 2021-01-29 OER102707 OER_Yingcheng_2101292151_P04 OEX012187 OES068741 30 2021-01-29 OER102708 OER_Yingcheng_2101292153_P05 OEX012187 OES068742 45 2021-01-29 OER102709 OER_Yingcheng_2101292154_P06 OEX012187 OES068743 24 2021-01-29 OER102710 OER_Yingcheng_2101292156_P7 OEX012187 OES068744 48 2021-01-29 OER102711 OER_Yingcheng_2101292157_P08 OEX012187 OES068745 36 2021-01-29 OER102712 OER_Yingcheng_2101292159_P09 OEX012187 OES068746 48 2021-01-29 OER102713 OER_Yingcheng_2101292200_P10 OEX012187 OES068747 36 2021-01-29 OER102714 OER_Yingcheng_2101292201_P11 OEX012187 OES068748 27 2021-01-29 OER102715 OER_Yingcheng_2101292202_P12 OEX012187 OES068749 6 2021-01-29
Author Information
- Create Date
- 2021-01-29
- Last Modified
- 2021-09-10
- Submission
- Yingcheng Wu
- Fudan University