General Information
- Project ID
- OEP003465
- Project Name
- IP-MS of FLAG-MRG15 in HEK 293T
- Description
- FLAG-MRG15 was overexpressed in HEK 293T cells, with the cell pellets collected after 48 hours and lysed with RIPA buffer (Millipore, 20188). FLAG-MRG15 was then immunoprecipitated with anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, M8823) at 4 °C overnight. The M2 beads were washed with RIPA buffer (Millipore, 20188) for three times, and binding proteins were eluted with 3× FLAG peptide (Sigma-Aldrich, F4799). The eluate from M2 beads was subjected to mass spectrometry analysis.
- Publications
-
MRG15 aggravates non-alcoholic steatohepatitis progression by regulating the mitochondrial proteolytic degradation of TUFM.
Yang Z, Chen H, Lu Y, Gao Y, Sun H, Wang J, Jin L, Chu J, Xu S. Genetic evidence of tri-genealogy hypothesis on the origin of ethnic minorities in Yunnan. BMC Biol. 2022 Jul 21;20(1):166.
(PMID: 35985547) (DOI: 10.1016/j.jhep.2022.07.017)
Project information
- Experiments
- 1
Experiment ID Experiment name Experiment type Create Date OEX018169 OEX_Cheng_2206211625 Proteomic 2022-06-21 - Samples
- 1
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES191026 IP-MS of MRG15 Human Homo sapiens cell line 2022-06-21 - Runs
-
1
Run ID Run Name Experiment Sample Data Num Create Date OER257727 OER_Cheng_2206211640 OEX018169 OES191026 1 2022-06-21
Author Information
- Create Date
- 2022-06-21
- Last Modified
- 2023-04-13
- Submission
- Cheng Tian
- Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences