To examine the distribution of Gli1-expressing cells in the skeletal muscle, we performed scRNA-seq using Gli1-CreERT2; R26-tdTomato mice, in which Gli1+ cells were labeled by tdTomato (tdT) after tamoxifen (TAM) induction. A total of 8,508 tdT+ (Gli1+) cells were sorted from hindlimb skeletal muscles of 3 adult male mice and pooled for scRNA-seq.
To further confirm the expression of Gli1 in the MuSC population, CD45-CD31-Sca1-Vcam1+ MuSCs were isolated from wild-type mice by fluorescence-activated cell sorting (FACS),and analyzed by scRNA-seq. As expected, the majority of the isolated MuSCs expressed Pax7