General Information
- Project ID
- OEP004110
- Project Name
- Opposing roles of the stromal architecture surrounding and occupying tumor mass in hepatocellular carcinoma
- Description
- We performed the first spatial multi-omics analysis of HCC to explore the potential mechanism underlying the opposing roles of the stromal architecture surrounding and occupying tumor mass. Using spatial proteomics, and high-resolution spatial transcriptomics (Stereo-seq) with paired single-cell/single-nucleus sequencing, single-cell chromatin profiling, and multi-plex imaging, we revealed two distinct fibroblast subpopulations, CAF1_FAP and CAF3_C7, coordinating with local TME cells to form inflammatory hubs in the tumor-occupying stroma and wound healing hubs in the tumor-surrounding stroma, respectively. Moreover, we validated the prognostic value of CAF1_FAP and CAF3_C7, and revealed their distinct ECM profiles, gene regulatory networks, and cell-cell interactome, providing insights for the design of novel stromal-targeted therapies in HCC. Our study proposes the balance between tumor-surrounding stroma and tumor-occupying stroma mediates tumor progression and anti-tumor immunity and highlights the potential of spatial multi-omics studies in identifying novel biomarkers and informing targeting strategies in HCC and other human cancer types beyond.
Project information
- Experiments
- 7
Experiment ID Experiment name Experiment type Create Date OEX023080 proteome Proteomic 2023-04-15 OEX023081 single-cell RNA-seq Transcriptomic single cell 2023-04-15 OEX023082 single-nucleus RNA-seq Transcriptomic single cell 2023-04-15 OEX023083 single-nucleus ATAC-seq Genomic single cell 2023-04-15 OEX023084 high-resolution spatial transcriptomics Transcriptomic single cell 2023-04-15 OEX026418 RNA-seq Transcriptomic 2023-12-18 OEX026419 CUT&Tag-seq Genomic 2023-12-18 - Samples
- 30
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES307514 C01 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307515 C02 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307516 C03 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307517 C04 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307518 C05 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307519 C06 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307520 C07 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307521 C09 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307522 C10 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307523 C11 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307524 C12 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307525 C13 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307526 C14 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307527 C15 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307528 N01 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307529 C08 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307530 N02 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307531 N03 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307532 N04 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307533 N05 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307534 N06 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307535 N07 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307536 N08 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307537 N09 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307538 N10 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307539 N11 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307540 N12 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307541 N13 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307542 N14 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 OES307543 N15 Human Homo sapiens Stroma/Tumor/Liver 2023-05-11 - Runs
-
30
Run ID Run Name Experiment Sample Data Num Create Date OER395362 Proteome-C05 OEX023080 OES307518 1 2023-05-11 OER395363 Proteome-C15 OEX023080 OES307527 1 2023-05-11 OER395364 Proteome-C01 OEX023080 OES307514 1 2023-05-11 OER395365 Proteome-C02 OEX023080 OES307515 1 2023-05-11 OER395366 Proteome-C07 OEX023080 OES307520 1 2023-05-11 OER395367 Proteome-C03 OEX023080 OES307516 1 2023-05-11 OER395368 Proteome-C06 OEX023080 OES307519 1 2023-05-11 OER395369 Proteome-C08 OEX023080 OES307529 1 2023-05-11 OER395370 Proteome-C09 OEX023080 OES307521 1 2023-05-11 OER395371 Proteome-C11 OEX023080 OES307523 1 2023-05-11 OER395372 Proteome-C04 OEX023080 OES307517 1 2023-05-11 OER395373 Proteome-C12 OEX023080 OES307524 1 2023-05-11 OER395374 Proteome-C14 OEX023080 OES307526 1 2023-05-11 OER395375 Proteome-C13 OEX023080 OES307525 1 2023-05-11 OER395376 Proteome-N03 OEX023080 OES307531 1 2023-05-11 OER395377 Proteome-N01 OEX023080 OES307528 1 2023-05-11 OER395378 Proteome-N02 OEX023080 OES307530 1 2023-05-11 OER395379 Proteome-N07 OEX023080 OES307535 1 2023-05-11 OER395380 Proteome-N04 OEX023080 OES307532 1 2023-05-11 OER395381 Proteome-N06 OEX023080 OES307534 1 2023-05-11 OER395382 Proteome-N08 OEX023080 OES307536 1 2023-05-11 OER395383 Proteome-N09 OEX023080 OES307537 1 2023-05-11 OER395384 Proteome-N11 OEX023080 OES307539 1 2023-05-11 OER395385 Proteome-N14 OEX023080 OES307542 1 2023-05-11 OER395386 Proteome-N13 OEX023080 OES307541 1 2023-05-11 OER395387 Proteome-N12 OEX023080 OES307540 1 2023-05-11 OER395388 Proteome-N15 OEX023080 OES307543 1 2023-05-11 OER395389 Proteome-C10 OEX023080 OES307522 1 2023-05-11 OER395390 Proteome-N05 OEX023080 OES307533 1 2023-05-11 OER395391 Proteome-N10 OEX023080 OES307538 1 2023-05-11 - Analysis
-
8
Analysis ID Analysis Title Analysis Type Data Date OEZ013906 SNV and somatic indel Other 1 2023-04-15 OEZ013907 Processed shotgun proteomic profiles Other 1 2023-04-16 OEZ013915 Processed_snRNA-seq Other 4 2023-05-11 OEZ013916 Processed_snATAC-seq Other 35 2023-05-11 OEZ013917 Processed single-cell spatial transcriptomics Other 24 2023-05-12 OEZ013918 Processed spatial transcriptomics data at bin50-level Other 39 2023-05-12 OEZ014525 Processed_CUT&Tag-seq data Other 3 2023-12-18 OEZ014526 Processed RNA-seq data Other 2 2023-12-18
Author Information
- Create Date
- 2023-04-15
- Last Modified
- 2023-12-18
- Submission
- Yifei Cheng
- Fudan University