General Information
- Project ID
- OEP004944
- Project Name
- LC-CNSm
- Description
- Our understanding of the underlying mechanisms for central nervous system metastasis (CNSm), including leptomeningeal metastasis (LM) and brain parenchymal metastasis (BM), is limited. To address this, we conducted single-cell RNA sequencing on 24 patients with CNSm to decipher and compare the tumor microenvironment (TME) of LM and BM. Our results reveal that the TME of LM is more immunosuppressive, characterized by reduced lymphocytes and an increased presence of SPP1+ myeloid cells compared to BM. Additionally, we observed TLS-like structures in a few BM samples, associated with a favorable response to TKI therapy. Furthermore, we explored the correlation between the endothelial cell subtype Endo2 and tumor volume in BM, and systematically compared blood-brain barrier (BBB) cells in CNSm to normal conditions, revealing significant phenotypic remodeling. These findings provide insights into the clinical differences between BM and LM and contribute to a better understanding and clinical treatment of CNSm.
Project information
- Experiments
- 2
- Samples
- 28
Sample ID Sample Name Sample Subject Type Organism Tissue Create Date OES354733 LM01 Human Homo sapiens Leptomeninge 2024-01-24 OES354734 BM01 Human Homo sapiens Brain parenchyma 2024-01-24 OES354735 BM02 Human Homo sapiens Brain parenchyma 2024-01-24 OES354736 LM02 Human Homo sapiens Leptomeninge 2024-01-24 OES354737 BM03 Human Homo sapiens Brain parenchyma 2024-01-24 OES354738 BM04 Human Homo sapiens Brain parenchyma 2024-01-24 OES354739 LM03 Human Homo sapiens Leptomeninge 2024-01-24 OES354740 BM05 Human Homo sapiens Brain parenchyma 2024-01-24 OES354741 LM04 Human Homo sapiens Leptomeninge 2024-01-24 OES354742 LM04p Human Homo sapiens Lung 2024-01-24 OES354743 BM06 Human Homo sapiens Brain parenchyma 2024-01-24 OES354744 BM06p Human Homo sapiens Lung 2024-01-24 OES354745 LM05 Human Homo sapiens Leptomeninge 2024-01-24 OES354746 BM07 Human Homo sapiens Brain parenchyma 2024-01-24 OES354747 BM07p Human Homo sapiens Lung 2024-01-24 OES354748 BM08 Human Homo sapiens Brain parenchyma 2024-01-24 OES354749 BM09 Human Homo sapiens Brain parenchyma 2024-01-24 OES354750 LM06 Human Homo sapiens Leptomeninge 2024-01-24 OES354751 LM06p Human Homo sapiens Lung 2024-01-24 OES354752 BM10 Human Homo sapiens Brain parenchyma 2024-01-24 OES354753 BM11 Human Homo sapiens Brain parenchyma 2024-01-24 OES354754 BM12 Human Homo sapiens Brain parenchyma 2024-01-24 OES354755 BM13 Human Homo sapiens Brain parenchyma 2024-01-24 OES354756 LM12 Human Homo sapiens Leptomeninge 2024-01-24 OES354757 BM14 Human Homo sapiens Brain parenchyma 2024-01-24 OES354758 BM15 Human Homo sapiens Brain parenchyma 2024-01-24 OES354759 LM14 Human Homo sapiens Leptomeninge 2024-01-24 OES354760 BM16 Human Homo sapiens Brain parenchyma 2024-01-24 - Runs
-
28
Run ID Run Name Experiment Sample Data Num Create Date OER455517 run_BM01 OEX027253 OES354734 36 2024-01-24 OER455518 run_BM02 OEX027253 OES354735 48 2024-01-24 OER455519 run_BM03 OEX027253 OES354737 64 2024-01-24 OER455520 run_BM04 OEX027253 OES354738 48 2024-01-24 OER455521 run_BM05 OEX027253 OES354740 64 2024-01-24 OER455531 run_BM13 OEX027253 OES354755 4 2024-01-24 OER455532 run_BM14 OEX027253 OES354757 2 2024-01-24 OER455533 run_BM15 OEX027253 OES354758 2 2024-01-24 OER455534 run_BM16 OEX027253 OES354760 2 2024-01-24 OER455535 run_LM01 OEX027253 OES354733 52 2024-01-24 OER455536 run_LM02 OEX027253 OES354736 64 2024-01-24 OER455537 run_LM03 OEX027253 OES354739 16 2024-01-24 OER455542 run_LM12 OEX027253 OES354756 4 2024-01-24 OER455543 run_LM14 OEX027253 OES354759 2 2024-01-24 OER455522 run_BM06 OEX027254 OES354743 2 2024-01-24 OER455523 run_BM06p OEX027254 OES354744 2 2024-01-24 OER455524 run_BM07p OEX027254 OES354747 2 2024-01-24 OER455525 run_BM07 OEX027254 OES354746 2 2024-01-24 OER455526 run_BM08 OEX027254 OES354748 2 2024-01-24 OER455527 run_BM09 OEX027254 OES354749 2 2024-01-24 OER455528 run_BM10 OEX027254 OES354752 2 2024-01-24 OER455529 run_BM11 OEX027254 OES354753 2 2024-01-24 OER455530 run_BM12 OEX027254 OES354754 2 2024-01-24 OER455538 run_LM04p OEX027254 OES354742 2 2024-01-24 OER455539 run_LM04 OEX027254 OES354741 2 2024-01-24 OER455540 run_LM05 OEX027254 OES354745 2 2024-01-24 OER455541 run_LM06p OEX027254 OES354751 2 2024-01-24 OER455544 run_LM06 OEX027254 OES354750 2 2024-01-24 - Analysis
-
5
Analysis ID Analysis Title Analysis Type Data Date OEZ014553 cellranger_expr Reference Alignment 1 2024-01-03 OEZ014554 cellranger_tcr Reference Alignment 1 2024-01-03 OEZ014555 celescope_expr Reference Alignment 1 2024-01-03 OEZ014556 cellranger_bcr Reference Alignment 1 2024-01-03 OEZ014581 seurat Reference Alignment 2 2024-01-24
Author Information
- Create Date
- 2024-01-03
- Last Modified
- 2024-01-25
- Submission
- shenbing shan
- Lingang Laboratory