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PROJ
The transcriptome sequencing of E. coli with AMPs
Description
The transcriptome sequencing of E. coli with AMPs
OEP005095
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PROJ
Stool 16s rRNA sequencing of mice with thigh infection
Description
Stool 16s rRNA sequencing of mice with thigh infection
OEP005091
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PROJ
N/A
Description
N/A
OEP005172
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PROJ
VLS 16S DATA in girls
Description
OEP005161
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PROJ
H3K4me3 ChIP-seq of CD4+T cell from SLE patients and HC
Description
OEP004403
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PROJ
ESCC_FUDAN
Description
OEP003180
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PROJ
Screening and identification of immunological signatures in cord blood of HIV-exposed uninfected infants
Description
In the course of immune development, HIV-exposed uninfected (HEU) infants exhibit abnormal immune function and higher infectious morbidity compared to HIV-unexposed uninfected (HUU) infants. Yet functional phenotypes and the regulators associated with in-utero HIV exposure remain largely obscure. Herein, we utilized flow cytometry and RNA-seq technologies to establish the immune and transcriptional profiling in cord blood from 9 HEU mother-infant pairs and 24 HUU pairs. On top of that, we compared the cord blood dataset with the maternal venous blood dataset to characterize the unique effects induced by in-utero HIV exposure. Flow cytometry immunophenotyping revealed a significant decrease in the level of B lymphocyte subsets in HEU cord blood compared to HUU cord blood. Expression profiling-based assessment of cell abundance indicated a marked reduction in naive B cells in HEU cord blood, supporting the altered composition of B lymphocyte subsets in HEU infants. Functional enrichment results demonstrated a propensity for suppressed innate immune responses and impaired immune regulatory function of B cells in HEU cord blood. Additionally, through a combination of differential expression analysis, co-expression network analysis, and feature selection analysis, we identified an immunologically significant HEU-related signature. This 4-gene signature could effectively assess B cell levels in cord blood, enabling discrimination between HEU and HUU infants. This study provides the first transcriptomic characterization of HEU cord blood compared to maternal venous blood and establishes a 4-gene-based classifier for predicting immunological abnormalities in HEU infants. These findings hold promise for uncovering new mechanisms underlying abnormal immune system development in infants due to in-utero HIV exposure.
OEP005165
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PROJ
Metabolic characterization of sphere-derived prostate cancer stem cells
Description
Alteration of cell metabolism is one of the essential characteristics of tumor growth. Cancer stem cells (CSCs) are the initiating cells of tumorigenesis, proliferation, recurrence, and other processes, and play an important role in therapeutic resistance and metastasis. Thus, identification of the metabolic profiles in prostate cancer stem cells (PCSCs) is critical to understanding prostate cancer progression. Here, using untargeted metabolomics and lipidomics methods, we showed distinct metabolic differences between prostate cancer cells and PCSCs. We obtained DU145 spheres by ultralow-attached culture in sphere conditional medium. We use serum-induced sphere (adherent state) as control (Adh), and generation 2 (G2) and generation 3 (G3) of spheres as experimental groups to study the metabolic changes between prostate cancer cells and cancer stem cells.
OEP004085
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PROJ
RNA-seq of photorespiratory bypass plants
Description
RNA-seq of photorespiratory bypass plants
Used IDNODEP00000033
OEP000073
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PROJ
Unveiling the Methionine Cycle: A Key Metabolic Signature and NR4A2 as a Methionine-Responsive Oncogene in Esophageal Squamous Cell Carcinoma
Description
RNA-seq and MeRIP-seq data
OEP004978