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  • PROJ Microbial community succession patterns in the Changjiang river-ocean continuum

    jiao liu, ocean university of china,2024.03.31

    Description

    we investigated the planktonic and benthic prokaryotic communities along a river-to-sea continuum of the Changjiang River, China, to explore their distinct spatial dynamics.

    OEP005174

  • PROJ Mice mammary gland adult

    Chunye LIU, SIBCB,2023.01.08

    Description

    Mammary glands from 8- to 12-week-old virgin female mice were isolated and single-cell suspension was obtained. Mammary stem cell enriched population (Lin-, CD24+, CD29hi) cells were isolated using BD FACSJazz. The purity of sorted population was routinely checked and ensured to be more than 95%. Total RNA from day-3 colonies cultured in the presence of PBS and IL1beta was extracted with Trizol according to the manufacturer’s instructions.

    OEP003835

  • PROJ Adult_Mammary glands_10x RNAseq

    Chunye Liu, SIBCB,2023.07.14

    Description

    For the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we specifically sorted 2000 Procr+ MaSCs (CD31-, CD45-, Ter119-, CD24+, CD29hi) and also enrich 20000 mammary epithelial cells (CD31-, CD45-, Ter119-, CD24+), 30000 mammary immune cells (CD45+) and enrich 10000 stromal cells which contain endothelial cells and mesenchymal cells (CD45-, Ter119-, CD24-) and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq. Filtering, alignment to the mm10 database and unique molecular identifier (UMI)-collapsing were performed using the Cell Ranger (v2) pipeline with default mapping arguments (10′ Genomics). Cell filtering: we used the Seurat 3 R package for data integration, analysis and visualization. To create Seurat object, genes which expressed in at least 3 cells and cells which had at least 200 detected genes, 1500 detected UMIs and no more than 5000 detected UMIs were selected. PC selection: the differentially expressed genes were found by “vst” method and the top 2000 differentially expressed genes were selected for PCA analysis. PCs selection was based on an elbow plot. 20 PCs were used for analysis. Dimensional reduction, cell clustering and data

    OEP004257

  • PROJ Adult_Mammary glands_10x RNAseq_1

    Chunye Liu, Shanghai Institutes for Biological Sciences,2023.04.14

    Description

    For the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we enriched 2000 Procr+ basal cells, 30000 mammary epithelial cells and 30000 mammary immune cells and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq.

    OEP004107

  • PROJ The transcriptome sequencing of E. coli with AMPs

    Lei Zhang, Shandong University,2024.02.29

    Description

    The transcriptome sequencing of E. coli with AMPs

    OEP005095

  • PROJ Stool 16s rRNA sequencing of mice with thigh infection

    Lei Zhang, Shandong University,2024.02.29

    Description

    Stool 16s rRNA sequencing of mice with thigh infection

    OEP005091

  • PROJ N/A

    zhangwen xiao, ,2024.03.29

    Description

    N/A

    OEP005172

  • PROJ VLS 16S DATA in girls

    Lan Yu, zhejiang University,2024.03.26

    Description

    OEP005161

  • PROJ H3K4me3 ChIP-seq of CD4+T cell from SLE patients and HC

    Delong Feng, The Second Xiangya hospital of Central South University,2023.08.11

    Description

    OEP004403

  • PROJ ESCC_FUDAN

    Liyun Yuan, Sibs,2022.02.28

    Description

    OEP003180