Jinxin Xu, Xiamen University,2023.03.08

Description

OEP003994

Jianxiang Shi, Zhengzhou University,2023.05.25

Description

Background BCMA CAR-T is highly effective for relapsed/refractory multiple myeloma(R/R-MM) and significantly improves the survival of patients. However, the short remission time and high relapse rate of MM patients treated with BCMA CAR-T remain bottlenecks that limit long-term survival. The immune microenvironment of the bone marrow (BM) in R/R-MM may be responsible for this. The present study aims to present an in-depth analysis of resistant mechanisms and to explore potential novel therapeutic targets for relapse of BCMA CAR-T treatment via single-cell RNA sequencing (scRNA-seq) of BM plasma cells and immune cells. Methods This study used 10X Genomic scRNA-seq to identify cell populations in R/R-MM CD45+ BM cells before BCMA CAR-T treatment and relapse after BCMA CAR-T treatment. Cell Ranger pipeline and CellChat were used to perform detailed analysis. Results We compared the heterogeneity of CD45+ BM cells before BCMA CAR-T treatment and relapse after BCMA CAR-T treatment. We found that the proportion of monocytes/macrophages increased, while the percentage of T cells decreased at relapse after BCMA CAR-T treatment. We then reclustered and analyzed the alterations in plasma cells, T cells, NK cells, DCs, neutrophils, and monocytes/macrophages in the BM microenvironment before BCMA CAR-T treatment and relapse after BCMA CAR-T treatment. We show here that the percentage of BCMA positive plasma cells increased at relapse after BCMA CAR-T cell therapy. Other targets such as CD38, CD24, SLAMF7, CD138, and GPRC5D were also found to be expressed in plasma cells of the R/R-MM patient at relapse after BCMA CAR-T cell therapy. Furthermore, exhausted T cells, TIGIT+NK cells, interferon-responsive DCs, and interferon-responsive neutrophils, increased in the R/R-MM patient at relapse after BCMA CAR-T cell treatment. Significantly, the proportion of IL1βhi Mφ, S100A9hi Mφ, interferon-responsive Mφ, CD16hi Mφ, MARCO hi Mφ, and S100A11hi Mφ significantly increased in the R/R-MM patient at relapse after BCMA CAR-T cell therapy. Cell–cell communication analysis indicated that monocytes/macrophages, especially the MIF and APRIL signaling pathway are key players in R/R-MM patient at relapse after BCMA CAR-T cell therapy. Conclusion Taken together, our data extend the understanding of intrinsic and extrinsic relapse of BCMA CAR-T treatment in R/R-MM patient and the potential mechanisms involved in the alterations of antigens and the induced immunosuppressive microenvironment, which may provide a basis for the optimization of BCMA CAR-T strategies. Further studies should be performed to confirm these findings.

OEP004164

Jianmin Zhang, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College,2023.05.24

Description

Idiopathic inflammatory myopathy (IIM) is a heterogeneous group of autoimmune diseases with various clinical manifestations, treatment responses, and prognoses. We applied MALDI-TOF-MS to examine the serum metabolome of 144 patients with IIM and analyze differentially expressed metabolites among IIM subgroups or MSA groups. Toal 180 samples were performed, and part of them were excluded from our study due to a lack of a diagnosis of IIM subgroups or MSA groups, or from the same patients. Raw data of 180 samples were kept.

OEP004162

Jianmin Zhang, Chinese Academy of Medical Sciences and School of Basic Medicine, Peking Union Medical College,2023.05.25

Description

For supporting the metabolite identification of differentially expressed peaks among IIM subgroups or MSA groups based on MALDI-TOF, we applied LC-MS on 5 mixed serum samples from IIM patients to perform metabolite identification as a reference database. Raw data were kept here and ddMS2 was performed on QC samples as shown.

OEP004163

Xu Ning, fudan university,2021.09.27

Description

Transcriptome and WES for UC

OEP002774

Huanhuan Wei, ,2023.05.23

Description

OEP004150

Liting Jiang, ,2023.05.15

Description

OEP004145

Yun Tian, Xiamen University,2020.12.02

Description

Microbial communities harbor keystone taxa, which are drivers of microbiome structure and functioning. However, the characterization and manipulation of such taxa is a major challenge due to the complexity of microbial communities and rapid turnover in both time and space. Here, mangrove sediment microcosms were set up as reductive experimental models to trigger and track the turnover of keystone taxa driven by changing environmental factors to address this challenge. Based on BaP-contaminated and (or) nitrate-added microcosms, shotgun metagenomic and 16S rRNA gene sequencing were launched.

OEP001343

Jianxiang Shi, Zhengzhou University,2023.05.05

Description

Bufalin is a main component in toad venom, which is derived from the dry secretions of the posterior auricular gland and skin gland of the Chinese toad. It has a strong inhibitory effect on various solid tumors, including liver cancer, pancreatic cancer, colorectal cancer, gastric cancer, and bladder cancer. Based on the actual needs of patients with inoperable esophageal squamous cell carcinoma (ESCC) in middle and advanced stage, this study intends to combine bufalin with radiotherapy to guide radiotherapy for middle and advanced ESCC patients and explore the mechanism of bufalin in enhancing the radiosensitivity of ESCC.

OEP004136

Yu Zhang, Shanghai Jiaotong University,2023.02.23

Description

The mock communities of MT-DeepSea are expected to improve standardization and quality assurance of metatranscriptomic analysis for deep-sea microbes.

OEP003879