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PROJ
Microbiome of different microhabitats in aquaponics and recirculating aquaculture systems
Description
OEP005493
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PROJ
Shuangliu GDM
Description
OEP003504
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PROJ
Metavirome samples from Shidou and Tingxi reservoirs since 2020
Description
Metavirome sequences from Shidou and Tingxi reservoirs stratification samples since 2020
OEP005384
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PROJ
Soil virome under drought
Description
Soil viruses within aggregates under drought.
OEP005425
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PROJ
High-resolution spatial transcriptomic atlas of human skin and its aging.
Description
OEP005458
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PROJ
Single cell sequencing technology reveals the correlation between B lymphocytes and vascular inflammatory symptoms of Kawasaki disease
Description
Kawasaki disease (KD) is the most common cause of acquired heart disease in children in developed countries. Although functional and phenotypic changes of immune cells have been reported, a global understanding of immune responses underlying acute KD is unclear. Here, using single-cell RNA sequencing, we profile peripheral blood mononuclear cells from six patients with acute KD before intravenous immunoglobulin therapy and from three age-matched healthy&fever controls.Patients were categorized into early and late vasculitis groups based on the duration of vasculitis symptoms at diagnosis and sampling, with the early vasculitis group having symptoms for less than 3 days and the late vasculitis group for 3 days or more. The study found a significant increase in the proportion of CD19+ B cells and their subsets during the acute phase of KD, particularly in patients with later onset of vasculitis symptoms. Functional enrichment analysis revealed significant changes in gene expression associated with B cell activation and immune response. Additionally, the genes S100A8, S100A9, and S100A12 were significantly upregulated during the early stages of vasculitis in KD.This study highlights the close relationship between the number of immune cells and gene expression changes with the duration of vasculitis in KD, providing new insights into the pathophysiological mechanisms of the disease and potential prognostic indicators for clinical treatment. These findings pave the way for further research and the development of therapeutic strategies for KD.
OEP005481
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PROJ
Diazotrophs in cold-seep bottom waters
Description
OEP005056
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PROJ
Single cell sequencing technology reveals the correlation between B lymphocytes and vascular inflammatory symptoms of Kawasaki disease
Description
OEP005475
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PROJ
Serum and tissue microbiome (2bRAD-M sequencing) of CRC
Description
This project is the investigation of the characteristics of serum and tissue microbiota between CRC patients using 2bRAD-M gene sequencing.
OEP004048
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PROJ
Co-regulation of alternative splicing and transcription initiation and termination revealed by long-read full-length RNA sequencing
Description
Alternative splicing regulation depends on the coupling between transcription and splicing. However, the interaction between alternative splicing and transcription initiation and termination has remained largely unknown due to limited technologies enabling simultaneous measurement. Here, we developed the longPASS approach to accurately identify and quantify transcription start sites (TSS) and polyadenylation sites (PAS) from long-read RNA sequencing data. Using it, we further constructed full-length transcript isoforms to systematically assess the co-regulatory relationship between alternative splicing and TSS/PAS usage. Our finding revealed thousands of TSS/PAS-associated splicing events (TASE) in human cell, representing a pervasive gene regulation mechanism that is consistent across different genetic perturbations and diverse human tissues.
OEP005259