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PROJ
test one-stop project
Description
one-stop project
OEP000773
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PROJ
Genome-wide CRISPR screens identify PKMYT1 as a therapeutic target in pancreatic ductal adenocarcinoma
Description
Pancreatic ductal adenocarcinoma (PDAC) is a devastating disease with an overall 5-year survival rate of <12% due to the lack of effective treatments. KRAS G12C and mutant-BRAC targeted therapies are actively investigated in PDAC patients. Unfortunately, only a small subgroup of patients harbors these mutants. Novel treatment strategies are urgently needed. Here, we identified through genome-wide CRISPR screens that PKMYT1 is a non-mutant, genetic vulnerability of PDAC, but not a pan-essential depending on TP53 mutation status. Higher PKMYT1 expression levels indicate poor prognosis in PDAC patients. PKMYT1 ablation inhibits tumor growth and proliferation in vitro and in vivo by regulating cell cycle progression and inducing apoptosis. Moreover, pharmacological inhibition of PKMYT1 shows efficacy in multiple PDAC cell models and effectively induces tumor regression without overt toxicity in PDAC cell line–derived xenograft and in more clinically relevant patient-derived xenograft models. Mechanistically, in addition to its canonical functions of phosphorylating CDK1, proteomic screens demonstrate that PKMYT1 interacts with PLK1 and PRKDC in PDAC. PKMYT1 inhibition regulates PLK1 and PRKDC expression and activation, respectively, to maximize treatment effects for PDAC. These results define PKMYT1 dependency in PDAC and identify potential therapeutic strategies for clinical translation.
OEP004116
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PROJ
Germline Variation in Gastric Cancer Patient
Description
OEP004125
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PROJ
Using formalin fixed paraffin embedded tissue to characterize the microbiota in p16-positive and p16-negative tongue squamous cell carcinoma
Description
In this study, to compare the microbiota compositions betweenp16-positive and p16-negative patients with tongue squamous cell carcinoma (TSCC), we performed a small pilot study of microbiological study of TSCC by paraffin tissue.
OEP003768
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PROJ
Chinese Quartet
Description
OEP000970
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PROJ
The children with sepsis for gut metagenome 16s rDNA sequencing
Description
OEP003851
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PROJ
CYLiu_mammary glands_10x RNAseq_1
Description
For the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we enriched 2000 Procr+ basal cells, 30000 mammary epithelial cells and 30000 mammary immune cells and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq.
OEP004107
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PROJ
Mice mammary gland adult
Description
Mammary glands from 8- to 12-week-old virgin female mice were isolated and single-cell suspension was obtained. Mammary stem cell enriched population (Lin-, CD24+, CD29hi) cells were isolated using BD FACSJazz. The purity of sorted population was routinely checked and ensured to be more than 95%. Total RNA from day-3 colonies cultured in the presence of PBS and IL1beta was extracted with Trizol according to the manufacturer’s instructions.
OEP003835
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PROJ
hydrosphere_wetland_metagenome
Description
Urban wetland biogeochemical cycling
OEP002472
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PROJ
urban wetland microbial community
Description
OEP001579