mujie zhang, ,2021.12.14

Description

OEP002984

mujie zhang, ,2022.06.28

Description

OEP003495

Cheng Tian, Shanghai Institute of Nutrition and Health, Chinese Academy of Sciences,2022.06.21

Description

FLAG-MRG15 was overexpressed in HEK 293T cells, with the cell pellets collected after 48 hours and lysed with RIPA buffer (Millipore, 20188). FLAG-MRG15 was then immunoprecipitated with anti-FLAG M2 Magnetic Beads (Sigma-Aldrich, M8823) at 4 °C overnight. The M2 beads were washed with RIPA buffer (Millipore, 20188) for three times, and binding proteins were eluted with 3× FLAG peptide (Sigma-Aldrich, F4799). The eluate from M2 beads was subjected to mass spectrometry analysis.

OEP003465

Shuhua Xu, PICB,2021.08.06

Description

This project is to investigate the genetic structure, population demography and adaptive evolution by analyzing the whole-exome sequences of 242 Western Yunnan minorities, including 65 Achang, 52 Dai, 65 Deang, and 60 Jingpo.

OEP002587

Shuhua Xu, PICB,2022.02.22

Description

Li people are the largest group of aboriginal islanders of China but remain under-investigated in both anthropology and genetics. We make the first whole-genome sequencing effort to gain insights into ancestral origins and evolutionary history of this islander group (HNL).

OEP003168

support scnode, ,2022.04.21

Description

Mouse Single-Cell Samples Collected by Shanghai General Hospital

OEP003311

Chenxu Gao, Shanghai Jiao Tong University Medical School Affiliated Ruijin Hospital,2023.04.11

Description

A prospective observational study enrolled 1,171 PDAC patients who had pancreatectomy is reported. After substantive follow-up and proteomic profiling of 191 patient samples, we identify functional modules with clinical relevance, establish a proteomic-level prognostic risk model for PDAC, and cross-validate the model using external as well as independent in-house cohorts. Two new biomarkers, indicative of PDAC adjuvant chemotherapy sensitivity, are discovered using integrated analysis of both clinical and proteomic datasets. Our prospective evidence points us towards a subgroup of patients to whom mild adjuvant chemo plan (for example S-1) might provide overall survival benefit.

OEP004100

Hongjun Li, Tsinghua University,2023.04.05

Description

For bulk RNA-seq, the plastic organoids and non-plastic organoids derived from KDL mouse model at early passages were collected. For bulk ATAC-seq, the time-series plastic organoids derived from KDL mouse model were collected for analysis. For single-cell RNA-seq (scRNA-seq) of KDL live time-series transition organoid cells, the organoid cells from cell culture at passage 1, 3 and 8 were collected for analysis.

OEP004095

Yu Yao, Fudan University,2023.04.05

Description

To establish a more clinically relevant model, we developed 3D bioprinted patient-derived glioma tissue (PDT) models. Surgically resected GBM tumor tissues were obtained from glioma patients with different demographic background and disease characteristics. Patient specimens underwent RNA-seq. Two patient specimens and their corresponding PDTs were also subjected to whole exosome sequencing and germline corrected using matched blood samples.

OEP004093

Jiexian Ma, Fudan university,2023.04.01

Description

To begin to elucidate the mechanism of action of decitabine with etoposide-based(D+E) treatment, we selected the TP53 mutation (THP-1 and U937) and TP53 WT (MOLM13 and OCI ) AML/MDS cell lines and treated them with etoposide and decitabine according to the indicated dose

OEP004090