Alteration of cell metabolism is one of the essential characteristics of tumor growth. Cancer stem cells (CSCs) are the initiating cells of tumorigenesis, proliferation, recurrence, and other processes, and play an important role in therapeutic resistance and metastasis. Thus, identification of the metabolic profiles in prostate cancer stem cells (PCSCs) is critical to understanding prostate cancer progression. Here, using untargeted metabolomics and lipidomics methods, we showed distinct metabolic differences between prostate cancer cells and PCSCs. We obtained DU145 spheres by ultralow-attached culture in sphere conditional medium. We use serum-induced sphere (adherent state) as control (Adh), and generation 2 (G2) and generation 3 (G3) of spheres as experimental groups to study the metabolic changes between prostate cancer cells and cancer stem cells.
scRNA-seq datasets of goat IVDs, including nucleus pulposus (NP), cartilage endplate (CEP), inner annulus fibrosus (iAF), outer annulus fibrosus (oAF).
Previously, Wakhi population were rarely analyzed. Here, we used 8 Wakhi samples to study.
The sediment collected from a black odorous river in the Dongbo community (Zhongtang town, Dongguan) was incubated in sediment microbial fuel cells in the laboratory. The response of microbial communities to the introduction of Lysinibacillus varians GY32 was investigated.
The transcriptomic profiles of Shewanella decolorationis S12 in the anaerobic reduction processes of iron and azo were detected and analysed to find out the key genes and explore the molecular mechanisms.
Effects of microbial fuel cell (MFC) on microbial community in the sediment of Pearl River Estuary were detected to illustrate the roles of MFC in environmental remediation
The transcriptomic profiles of Shewanella decolorationis S12 in microbial fuel cells added with flavins (RF and FMN) were detected and analysed to explore the promoting effects and molecular mechanisms of flavins on the extracellular electron transfer processes.
Metagenomic analysis on Futian Mangrove Sediment by lllumina sequencing.
Metagenomic analysis on Futian Mangrove Sediment by PacBio SMRT sequencing.
Brain are complex biological tissues which function relies on coordinated anatomical and molecular structure comprised by a large number of specialized cells. The spatial architecture of brain which is key to the understanding of its physiological and pathological significance is formed during embryo development. However, the molecular basis for discrete neuroanatomical domains particularly in the context of spatial organization of the brain is inadequate. Here, we introduced microfluidic indexing based spatial ATAC and RNA sequencing (MISAR-seq), a method for parallel profiling of gene expression and chromatin accessibility with spatial information retained in developing mouse brain. Our study has established a direct means to spatially determine the coordination between chromatin accessibility and transcriptome, identified the chromatin potential to define cell fate determination of brain structure, and uncovered spatiotemporal regulatory principles during mammalian brain development.