Notice: Private records are filtered that would not be displayed.
  • PROJ xu bioreactor 2022

    志杰 陈, East China Normal University,2022.04.27



  • PROJ xu bioreactor 2022

    志杰 陈, East China Normal University,2022.04.29



  • PROJ Viral-bacterial incubation experiment

    Cong Liu, East China Normal University,2023.03.16


    we conducted incubation experiment starting with mixing different amounts of soil viruses with soil bacteria and tracked the dynamics of virus and bacterial cell abundances as well as bacterial community composition.


  • PROJ Denitrification of CA

    志杰 陈, East China Normal University,2022.07.11



  • PROJ SCUBE2 mediates bone metastasis of luminal breast cancer by modulating immune-suppressive osteoblastic niches

    Guohong Hu, Shanghai Institute of Nutrition and Health,Chinese Academy of Sciences,2021.09.07


    Estrogen receptor (ER)-positive luminal breast cancer is a subtype with generally lower risk of metastasis to most distant organs. However, bone recurrence occurs preferentially in luminal cancer. The mechanisms of the subtype-specific organotropism remain elusive. Here we show that an ER-regulated secretory protein SCUBE2 contributes to bone tropism of luminal breast cancer. Single-cell RNA sequencing analysis reveals osteoblastic enrichment by SCUBE2 in early bone-metastatic niches. SCUBE2 facilitates release of tumor membrane-anchored SHH to activate Hedgehog signaling in mesenchymal stem cells, thus promoting osteoblast differentiation. Osteoblasts deposit collagens to suppress NK cells via the inhibitory LAIR1 signaling and promote tumor colonization. SCUBE2 expression and secretion are associated with osteoblast differentiation and bone metastasis in human tumors. Targeting Hedgehog by Sonidegib and targeting SCUBE2 with a neutralizing antibody both effectively suppress bone metastasis in multiple metastasis models. Overall, our findings provides an explanation for bone preference in luminal breast cancer and new approaches for metastasis treatment.


  • PROJ Study on vaginal bacteriome and mycobiome

    Lei Zhang, Shandong University,2021.11.14


    we recruited 114 RTI subjects (including 37 BV patients, 44 VVC patients and 33 UU patients) and 47 healthy controls. we conducted bacteria16S rDNA gene sequencing and fungal internal transcribed spacer (ITS)gene sequencing to analyze those 161 vaginal swabs samples.


  • PROJ An inflammatory checkpoint generated by IL1RN splicing offers therapeutic opportunity for KRAS mutant intrahepatic cholangiocarcinoma

    qiang gao, Department of Liver Surgery and Transplantation, Liver Cancer Institute, Zhongshan Hospital,2022.11.20


    KRAS mutations are causally linked to pro-tumor inflammation and identified as driving factors in tumorigenesis. Here, using multi-omics data gathered from a large set of patients, we showed that KRAS mutation was associated with a specific landscape of alternative mRNA splicing that connected to myeloid inflammation in intrahepatic cholangiocarcinoma (iCCA). Then, we identified a negative feedback mechanism in which the upregulation of interleukin 1 receptor antagonist (IL1RN)-201/203 due to alternative splicing confers vital anti-inflammatory effects in KRAS mutant iCCA. In KRAS mutant iCCA mice, both IL1RN-201/203 upregulation and anakinra treatment ignited a significant anti-tumor immune response by altering neutrophil recruitment and phenotypes. Furthermore, anakinra treatment synergistically enhanced anti-PD-1 therapy to activate intratumoral GZMB+ CD8+ T cells in KRAS mutant iCCA mice. Clinically, we found that high IL1RN-201/203 levels in KRAS mutant iCCA patients were significantly associated with superior response to anti-PD-1 immunotherapy.


  • PROJ Microbial community succession during a DHPS-addition incubation experiment

    Xiaofeng Chen, State Key Laboratory of Marine Environmental Science,2021.06.28


    Surface seawater sample was collected from Xiamen Bay (N 24°30.0688’, E 118°14.3181’), China, and prefiltered through 3 μm polycarbonate filters (142 mm, Millipore, USA) to remove large particles and grazers, but maintaining the prokaryotic community. The filtrate was then divided into 25 L polycarbonate bottles. DHPS was added into the seawater at a final concentration of 1 mM for DHPS treatments, while those with no additional substrate served as controls. Each treatment was conducted in triplicate. The incubation was conducted in the dark at room temperature. Bacteria were collected by filtering samples (0.5-1 L) through a 0.2 μm polycarbonate filters (47 mm, Millipore, USA) to determine the bacterial community.


  • PROJ Metagenome and metaproteome of bacteria in a shallowwater hydrothermal field (Kueishantao Islet, NE Taiwan)

    Xiaofeng Chen, State Key Laboratory of Marine Environmental Science,2020.12.01


    Metagenome and metaproteome of bacteria in a shallowwater hydrothermal field (Kueishantao Islet, NE Taiwan). Samples were collected in 2017, 2018 and 2019.


  • PROJ Proteomic characteristics of three Roseobacter strains, DSS-3, DFL12 and OCh114, gowing in DHPS and acetate

    Xiaofeng Chen, State Key Laboratory of Marine Environmental Science, Fujian Key Laboratory of Marine Carbon Sequestration,2020.12.01


    Label-free quantitatitve proteomics was used to identify changes in the proteome that occurs when Ruegeria pomeroyi DSS-3, Dinoroseobacter shibae DFL 12 and Roseobacter denitrificans OCh 114, grown in DHPS and acetate.