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PROJ
Hierarchically assembled helical fiber integrates effectively with bones
Description
We discovered both hierarchical channels and carbon component of HHF activated certain signaling pathways that promoted osteogenesis.
OEP002944
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PROJ
Global deep-sea sediment viromes 2.0
Description
OEP003844
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PROJ
Single-cell sequencing of immune cells after marathon and symptom-limited cardiopulmonary exercise
Description
Three participants were enrolled for CPX physical exercise testing and serial peripheral blood collection while another three participants were enrolled for serial peripheral blood collection before, during and after marathon. Intravenous blood samples were collected before the physical exercise as baseline and during the recovery period at 5 min, 60 min and 4 hours for CPX or 24 hours for marathon after the end of the physical exercise. In-depth profiling of single-cell RNA transcriptomics on isolated PBMCs was performed. After data curation and annotation, we obtained a total of 275,054 single-cell transcriptomes with information on expression of 23,494 genes.
OEP003910
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PROJ
Lineage plasticity of basal intermediate cell population in prostate homeostasis, inflammation and cancer initiation
Description
Many glandular epithelia are mainly comprised of basal cells and luminal cells, including prostate gland. Adult prostate basal and luminal cells are independently self-sustained by unipotent stem cells that can reactivate multipotency under prostate inflammation and carcinogenesis contexts. However, the defined basal stem cell populations responsible for prostate regeneration and their cell fates in prostate homeostasis, inflammation and carcinogenesis remain unclear. Using a genetic proliferation tracer (ProTracer) system, we found that basal cells had extensive cell loss and proliferation during androgen-mediated prostate regression and regeneration. A rare intermediate basal cell population that expresses luminal cell markers (Nkx3.1 and Pbsn) (termed Basal-B) and a large basal cell population (termed Basal-A) were identified in mouse prostates by single-cell RNA sequencing. Basal-B cells exhibited greater capacity for organoid formation and luminal cell differentiation in vitro. Genetic lineage tracing using dual recombinases showed that prostate homeostasis and regeneration are not driven by specific basal cell types. Fate-mapping results showed that Basal-B cells had the better tendency to generate luminal cells under bacteria-induced prostate inflammation. Deletion of Pten in basal cells resulted in Basal-A-to-Basal-B-to-luminal transition and prostatic intraepithelial neoplasia. Moreover, h-Basal-B cell population was significantly increased in human benign prostate hyperplasia and prostatic intraepithelial neoplasia compared with normal prostates. This study identifies intermediate Basal-B cells as potential stem cell population and provides genetic evidence of a bona fide of prostate basal cell lineage plasticity under physiological and pathological contexts.
OEP003997
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PROJ
Spatial omics study of human liver and lung
Description
OEP003435
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PROJ
Effect of nitrogen addition on mineralization of organic carbon in lake sediments of Qinghai-Tibet Plateau
Description
To explore how the bacterial, archaeal and fungal communities respond to nitrogen addition in lake sediment of Qinghai-Tibet Plateau
OEP003899
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PROJ
Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma
Description
Enhanced glycolysis and accumulation of lactate is a common feature in various types of cancer. Intracellular lactate drives a recently described type of posttranslational modification, lysine lactylation (Kla), on core histones. However, the impact of lactylation on biological processes of tumour cells remains largely unknown. Here we show a global lactylome profiling on a prospectively collected hepatitis B virus-related hepatocellular carcinoma (HCC) cohort. Integrative lactylome and proteome analysis of the tumours and adjacent livers identifies 9,275 Kla sites, with 9,256 sites on non-histone proteins, indicating that Kla is a prevalent modification beyond histone proteins and transcriptional regulation. Notably, Kla preferentially affects enzymes involved in metabolic pathways, including the tricarboxylic acid cycle, and carbohydrate, amino acid, fatty acid and nucleotide metabolism. We further verify that lactylation at K28 inhibits the function of adenylate kinase 2, facilitating the proliferation and metastasis of HCC cells. Our study therefore reveals that Kla plays an important role in regulating cellular metabolism and may contribute to HCC progression.
OEP002852
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PROJ
RNAseq of ARP1 cell line treated with or without NH4Cl
Description
RNAseq of ARP1 cell line treated with or without NH4Cl [1 mM].
OEP003891
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PROJ
Metagenomic sequencing of fecal samples from multiple myeloma patients at diagnosis and recurrence
Description
Metagenomic sequencing of fecal samples from multiple myeloma patients at diagnosis and recurrence
OEP002396
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PROJ
RNA-Seq data of human skeletal muscle and scWAT
Description
OEP003781