HelenHe Zhu, State Key Laboratory of Oncogenes and Related Genes, Renji‐Med‐X Stem Cell Research Center, Department of Urology, Ren Ji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China,2022.09.16

Description

The spleen plays a critical role in the pathogenesis of leukemia. However, our understanding of the splenic niche in regulating leukemic cells is very limited. Herein, we report identification of such a unique splenic niche population. Induced expression of a secreted protein Gremlin1 in a genetically engineered mouse model restrains the disease progression of chronic myeloid leukemia (CML) and synergizes with first-line tyrosine kinase inhibitor (TKI) treatment, whereas blockade of the Gremlin1 protein by a monoclonal antibody enhances the CML development. Intriguingly, the CML-promoting effect of anti-Gremlin1 antibody is most evident in the spleen but not in the bone marrow, which can be abrogated by splenectomy. Genetic ablation of Gremlin1+ cells leads to an accelerated CML progression. Together with analysis of single cell RNA-seq data, we find that Gremlin1 marks a unique stromal cell population in the spleen of not only mice, but also humans. Mechanistically, Gremlin1 induces apoptosis of leukemia stem cells (LSCs) via antagonizing the BMP pathway. Together, we demonstrate that Gremlin1 and Gremlin1+ cells are key defensive niche components in the spleen to limit the progression of CML, revealing an unprecedented mechanism for the body to fight off leukemia.

OEP003642

Tuantuan Gui, Shanghai JiaoTong University,2024.03.25

Description

Acute lymphoblastic leukemia (ALL) poses formidable challenges in adult patients, considering its heterogeneous nature and relatively unfavorable prognosis among hematological malignancies.The metabolic interplay between body organs and bone marrow (BM) in ALL remains poorly understood, with limited knowledge beyond BM microenvironment in promoting ALL or how the body responds.

OEP005155

Luo Ruijin, ,2023.11.09

Description

武汉开发部分-测试提交

OEP004707

Luo Ruijin, ,2024.04.09

Description

OEP005194

jiao liu, ocean university of china,2024.03.31

Description

we investigated the planktonic and benthic prokaryotic communities along a river-to-sea continuum of the Changjiang River, China, to explore their distinct spatial dynamics.

OEP005174

Chunye LIU, SIBCB,2023.01.08

Description

Mammary glands from 8- to 12-week-old virgin female mice were isolated and single-cell suspension was obtained. Mammary stem cell enriched population (Lin-, CD24+, CD29hi) cells were isolated using BD FACSJazz. The purity of sorted population was routinely checked and ensured to be more than 95%. Total RNA from day-3 colonies cultured in the presence of PBS and IL1beta was extracted with Trizol according to the manufacturer’s instructions.

OEP003835

Chunye Liu, SIBCB,2023.07.14

Description

For the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we specifically sorted 2000 Procr+ MaSCs (CD31-, CD45-, Ter119-, CD24+, CD29hi) and also enrich 20000 mammary epithelial cells (CD31-, CD45-, Ter119-, CD24+), 30000 mammary immune cells (CD45+) and enrich 10000 stromal cells which contain endothelial cells and mesenchymal cells (CD45-, Ter119-, CD24-) and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq. Filtering, alignment to the mm10 database and unique molecular identifier (UMI)-collapsing were performed using the Cell Ranger (v2) pipeline with default mapping arguments (10′ Genomics). Cell filtering: we used the Seurat 3 R package for data integration, analysis and visualization. To create Seurat object, genes which expressed in at least 3 cells and cells which had at least 200 detected genes, 1500 detected UMIs and no more than 5000 detected UMIs were selected. PC selection: the differentially expressed genes were found by “vst” method and the top 2000 differentially expressed genes were selected for PCA analysis. PCs selection was based on an elbow plot. 20 PCs were used for analysis. Dimensional reduction, cell clustering and data

OEP004257

Chunye Liu, Shanghai Institutes for Biological Sciences,2023.04.14

Description

For the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we enriched 2000 Procr+ basal cells, 30000 mammary epithelial cells and 30000 mammary immune cells and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq.

OEP004107

Lei Zhang, Shandong University,2024.02.29

Description

The transcriptome sequencing of E. coli with AMPs

OEP005095

Lei Zhang, Shandong University,2024.02.29

Description

Stool 16s rRNA sequencing of mice with thigh infection

OEP005091