DescriptionAcute lymphoblastic leukemia (ALL) poses formidable challenges in adult patients, considering its heterogeneous nature and relatively unfavorable prognosis among hematological malignancies.The metabolic interplay between body organs and bone marrow (BM) in ALL remains poorly understood, with limited knowledge beyond BM microenvironment in promoting ALL or how the body responds.
OEP005155
Description武汉开发部分-测试提交
OEP004707
Description
OEP005194
Descriptionwe investigated the planktonic and benthic prokaryotic communities along a river-to-sea continuum of the Changjiang River, China, to explore their distinct spatial dynamics.
OEP005174
DescriptionMammary glands from 8- to 12-week-old virgin female mice were isolated and single-cell suspension was obtained. Mammary stem cell enriched population (Lin-, CD24+, CD29hi) cells were isolated using BD FACSJazz. The purity of sorted population was routinely checked and ensured to be more than 95%. Total RNA from day-3 colonies cultured in the presence of PBS and IL1beta was extracted with Trizol according to the manufacturer’s instructions.
OEP003835
DescriptionFor the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we specifically sorted 2000 Procr+ MaSCs (CD31-, CD45-, Ter119-, CD24+, CD29hi) and also enrich 20000 mammary epithelial cells (CD31-, CD45-, Ter119-, CD24+), 30000 mammary immune cells (CD45+) and enrich 10000 stromal cells which contain endothelial cells and mesenchymal cells (CD45-, Ter119-, CD24-) and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq. Filtering, alignment to the mm10 database and unique molecular identifier (UMI)-collapsing were performed using the Cell Ranger (v2) pipeline with default mapping arguments (10′ Genomics). Cell filtering: we used the Seurat 3 R package for data integration, analysis and visualization. To create Seurat object, genes which expressed in at least 3 cells and cells which had at least 200 detected genes, 1500 detected UMIs and no more than 5000 detected UMIs were selected. PC selection: the differentially expressed genes were found by “vst” method and the top 2000 differentially expressed genes were selected for PCA analysis. PCs selection was based on an elbow plot. 20 PCs were used for analysis. Dimensional reduction, cell clustering and data
OEP004257
DescriptionFor the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we enriched 2000 Procr+ basal cells, 30000 mammary epithelial cells and 30000 mammary immune cells and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq.
OEP004107
DescriptionThe transcriptome sequencing of E. coli with AMPs
OEP005095
DescriptionStool 16s rRNA sequencing of mice with thigh infection
OEP005091
DescriptionN/A
OEP005172