General Information
- Sample ID
- OES002912
- Sample Name
- 0523
- Description
- Fibroblast donor cell (0523):Primary fibroblasts were isolated from a 61 day-old freshly aborted cynomolgus fetus. The fetal tissue were cut into small pieces after removing the head, tail, limbs, and viscera and digested with DNase (1 mg/ml) and collagenase IV (0.5 mg/ml) in cell culture medium containing DMEM supplemented with 100 IU/ml penicillin and streptomycin, 10% FBS, non-essential amino acids, and glutamine at 37C in 5% CO2 for 4 h. Isolated monkey fibroblasts were cultured in 10 cm dishes for 10-20 h until they reached confluency, disaggregated by trypsin, and stored in liquid nitrogen in cell culture medium containing 10% dimethylsulfoxide. Frozen fibroblasts (P0-P5) were thawed and cultured until confluency, and used for SCNT 3-7 days later.
- Subject type
- Animalia
- Used ID
- NODES03347312  
Sample information
- Organism
- Macaca fascicularis
- Projects
-
1
Project ID Project name Submitter Date OEP000101 SNP and STR Analysis of "ZZ" and "HH" Generated by SCNT Using Fetal Fibroblasts Qiang, Sun 2018-02-03 - Experiments
-
2
Experiment ID Experiment name Experiment type Experiment protocol Date OEX000102 SNP Analysis_ZZ_HH Genomic Ear tissue sample collected from monkeys were used to extract DNA. PCR with specific primers (F: CCACTTCACATCAAACCATCACTT, R: CAAGCAGCGAATACCAGCAAAA) in mtDNA were performed. DNA was amplified with 35 cycles of 95℃ for 30 s, 55℃ for 30 s, and 72℃ for 1 min, followed by a 5-min extension at 72℃. The PCR products were used for sequencing and the result were used for the SNP analysis. 2018-02-03 OEX000103 STR Analysis_ZZ_HH Genomic Ear tissue sample collected from monkeys were used to extract DNA. Locus-specific primers each containing a fluorescent dye (FAM/HEX/TMR) were used for PCR amplification in batches. FAM, HEX or TMR-labeled STR amplicons were diluted and mixed with internal size standard ROX500 and deionized formamide, followed by capillary electrophoresis on ABI PRISM 3730 genetic analyzer to obtain the raw data. 2018-02-03 - Runs
- 2
- Analysis
-
1
Analysis ID Analysis Title Analysis Type Data Date OEZ000367 STR analysis result of 0523 Other 0 2018-02-03
Author Information
- Create Date
- 2018-02-03
- Submission
- Sun Qiang,the Institute of Neuroscience (ION)