General Information
Sample information
- Organism
- Mus musculus
- Projects
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1
Project ID Project name Submitter Date OEP000195 Cytosine base editor generates substantial off-target single nucleotide variants in mouse embryos Yang, Hui 2018-11-30 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX001547 BE3ZEW Genomic The mixture of Cre, Cas9/BE3/ABE7.10 and sgRNA was injected into one blastomere of a 2-cell embryo, derived from Ai9 male mice mating with wild-type female mice. The action of Cre is expected to generate a chimeric embryo with half of cells labeled with tdTomato. The tdTomato+ cells and tdTomato- cells were isolated from the chimeric embryo at E14.5 by FACS and used for WGS respectively. The off-target SNVs and indels were identified by comparing the tdTomato+ cells with tdTomato- cells using three variant calling algorithms as indicated (Mutect2, Lofreq and Strelka for SNVs, and Mutect2, Scalpel and Strelka for indels). 2018-11-30 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER025234 B23 OEP000195 OEX001547 OES013954 2 2018-11-30 - Analysis
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1
Analysis ID Analysis Title Analysis Type Data Date OEZ000422 B23 Other 2 2018-12-19
Author Information
- Create Date
- 2018-11-30
- Submission
- Hui Yang,Shanghai Institutes for biological science, chinese academy of science