OEX003179
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mRNA-seq of COVID-19 patients |
Transcriptomic |
Total RNA from whole blood samples was extracted using the RNeasy Plus Universal Mini Kit (Qiagen) following the manufacturer’s introductions. The quantity and quality of RNA solution was assessed using a Qubit Flex fluorometer (Invitrogen) and an Agilent Bioanalyzer (Agilent Technologies) before library construction and sequencing. RNA library construction was performed as described in the VAHTS Universal V6 RNA-seq Library Prep Kit for Illumina (Vazyme, China). Ribosomal, globin and RN7S RNAs were depleted using the specially designed probes (Vazyme, China).
After final library quantification using a Qubit Flex fluorometer (Invitrogen) and quality control using the Bioptic Qsep100 to confirm the expected size distributions, all libraries (RNA and exRNA) were pair-end (150-bp reads) sequenced on the Illumina NovaSeq6000 platform (Illumina). |
2020-04-27 |