General Information
- Sample ID
- OES251606
- Sample Name
- E135_sec8
- Subject type
- Animalia
Sample information
- Organism
- Mus musculus
- Tissue
- whole embryo
- ATTRIBUTES
-
health_status healthy dev_stage embryonic day 13.5 age 13.5d - Projects
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1
Project ID Project name Submitter Date OEP003721 Three-dimensional molecular architecture of mouse organogenesis Peng, Guangdun 2022-10-30 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX021031 Spatial transcriptome Transcriptomic Organogenesis stage (embryonic day 13.5, E13.5) embryos were collected of C57BL/6 mice, and embryos images were taken for recording and conformation of developmental staging. Collected embryos were embedded in Tissue freezing medium and stored at -80°C until sectioning. The whole embryo tissue was serially cryo-sectioned along craniocaudal axis at 10μm, and about 1000 sections were harvested. Considering the morphology and uniform sampling, 10 sections were selected for spatial transcriptomic analysis by 10x Genomics Visium platform. The resulting cDNA were synthesized, amplificated and then purified using AMpure beads and size-selected approximately 300-500bp. The cDNA library was assessed by Qubit 4.0 Fluorometer and Qsep100 Bio-Fragment Analyzer (Bioptic). The cDNA libraries were sequenced on Illumina Novaseq 6000 system with paired-end 150 bp reads, aiming for 100k raw reads per spot. 2022-11-01 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER295205 E135_sec8 OEP003721 OEX021031 OES251606 3 2022-11-01 - Analysis
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1
Analysis ID Analysis Title Analysis Type Data Date OEZ013173 Processing Other 0 2022-11-01
Author Information
- Create Date
- 2022-11-01
- Submission
- Guangdun Peng,