General Information
- Sample ID
- OES315986
- Sample Name
- Adult_MG_1
- Description
- After subjecting the anesthetized mice to heart perfusion with PBS to remove peripheral blood, the lymph nodes in the mammary tissue were extracted. Following this, the fat pads were isolated from 8-week-old nulliparous or other specified-stage female mice.
- Subject type
- Animalia
Sample information
- Organism
- Mus
- Tissue
- Mammary gland
- Projects
-
1
Project ID Project name Submitter Date OEP004257 Adult_Mammary glands_10x RNAseq Liu, Chunye 2023-07-14 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX024424 Adult_MG Transcriptomic For the primary mammary gland scRNA-seq, Wild type (C57BL/6) mice were used. After preparation of primary mammary single cell suspension, we specifically sorted 2000 Procr+ MaSCs (CD31-, CD45-, Ter119-, CD24+, CD29hi) and also enrich 20000 mammary epithelial cells (CD31-, CD45-, Ter119-, CD24+), 30000 mammary immune cells (CD45+) and enrich 10000 stromal cells which contain endothelial cells and mesenchymal cells (CD45-, Ter119-, CD24-) and resuspended total cells at a density of 1000 cells per μl. And then 13,000 single cells were loaded for 10x single cell genomics RNA seq. 2023-07-14 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER422647 Adult_1 OEP004257 OEX024424 OES315986 8 2023-07-14
Author Information
- Create Date
- 2023-07-14
- Last Modified
- 2023-07-14
- Submission
- Chunye Liu,SIBCB