General Information
- Sample ID
- OES324302
- Sample Name
- radish
- Subject type
- Plantae
Sample information
- Organism
- Raphanus sativus
- Tissue
- leaf
- Treatment
- NA
- Sample loc
- Shanghai Chenshan Botanical Garden
- Projects
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1
Project ID Project name Submitter Date OEP004638 RefMetaPlant: a reference metabolome database for plants across five major phyla Li, Xuan 2023-10-12 - Experiments
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1
Experiment ID Experiment name Experiment type Experiment protocol Date OEX025249 LC-MS based untargeted metabolome analysis of plants Metabolomic The freeze-dried tissues were ground using a tissue grinder for 2 min at 55 Hz. 150 mg of powder was weighed and mixed with 1.5 ml 70% aqueous methanol (containing 1 mg/L capsaicin as the internal standard). The samples were vortexed every 10 min for 3 times and extracted overnight at 4 °C. Following centrifugation at 12000 g for 15 min, all the supernatants were filtered using a 0.22 μm filter and transferred to a liquid phase bottle for subsequent analysis. Samples were analyzed on a Waters Acquity Ultra Performance Liquid Chromatography system equipped with an ACQUITY UPLC BEH C18 column (1.7 μm, 2.1 mm × 100 mm) coupled with Q Exactive hybrid Q-Orbitrap-high resolution mass spectrometry (Q-Orbitrap-HRMS) (Thermo Fisher Scientific). 2023-10-12 - Runs
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1
Run ID Run Name Project Experiment Sample Data Date OER430856 OER430674 OEP004638 OEX025249 OES324302 1 2023-10-12
Author Information
- Create Date
- 2023-10-12
- Last Modified
- 2023-10-12
- Submission
- Xuan Li,Institute of Plant Physiology and Ecology